铅损伤对新生鼠脑组织中多胺的影响  

Influence of Pb on Polyamine Level in Neonatal Rat Brain

作　　者：雷洁[1] 古桂雄[1] 蒋滢[2] 黄美英[2] 朱赓伯[2] 朱玲俐[1] 

机构地区：[1]苏州大学附属儿童医院 [2]苏州大学基础医学院

出　　处：《氨基酸和生物资源》2002年第2期66-68,共3页Amino Acids & Biotic Resources

基　　金：江苏省社会发展基金资助课题 (BS2 0 0 0 0 36 )

摘　　要：将新生Wistar大鼠按腹腔注射醋酸铅的剂量 ,随机分为正常、低铅组、高铅组。各组注射剂量分别为 0 ,2 0 ,6 0mg/kg。定期测量体重 ,采用薄板层析法测脑中精脒的含量。结果显示正常组出生后体重增长迅速 ;与正常组相比 ,高、低铅组分别于出生 5、10天后体重增长明显落后 ;脑重、脑精脒含量均显著低于正常组 ,并且与铅损伤的剂量有明显的负相关 (r =- 0 .793,P <0 .0 1)。Neonatal Wistar rats were divided into the control group, low Pb group and high Pb group, and Pb 3(AC) 2 at doses of 0,20,60 mg/kg and respectively. The weight was measured, and the spedimine level of the brain was determined with the thin-layer-chromatography. The growth of weight in the control group was rapid, but in Pb-exposed groups was slow. Compared with the control group, the growth of weight in the high and low Pb groups was significantly lower after postnatal 5,10 days; brain weight and brain Spedimine were all significantly decreased; and the spedimine level and Pb exposure dose had negative relativity (r=-0.793, p<0.01). The data showed that the polyamine of brain was an useful index for the degree of Pb impairment.

关 键 词：铅损伤 新生鼠 脑组织 多胺 精脒 神经毒性 

分 类 号：R995[医药卫生—毒理学] R595.2[医药卫生—药学]