激素对盾叶薯蓣愈伤组织细胞生长和薯蓣皂甙元合成的调控  

Effect of Homones on Callus Growth and Diosgenin Synthesis in Callus of Dioscorea zingiberensis C.H.Wright

作　　者：李明芳[1] 刘选明[2] 刘斌[1] 刘俊[1] 

机构地区：[1]湖南大学生物技术研究院 [2]湖南大学

出　　处：《中国生物工程杂志》2002年第3期71-73,共3页China Biotechnology

基　　金：湖南省科技厅资助项目 (99NXY10 0 1 0 3 )

摘　　要：不同生长素对盾叶薯蓣 (DioscoreazingiberensisC .H .Wright)愈伤组织细胞生长量的影响差异是NAA >IAA >2 ,4 D ,而对薯蓣皂甙元合成的影响是 2 ,4 D >IAA >NAA。在有生长素存在的条件下 ,光照 2 4h ,另加 0 5mg LKT促进愈伤组织细胞的生长 ,而附加 0 5mg LBA促进薯蓣皂甙元的合成 ;相反 ,光照 12h ,另加 0 5mg LBA促进生长 ,附加 0 5mg LKT促进合成。愈伤组织生长越旺 ,薯蓣皂甙元积累越少。低浓度的生长素促进薯蓣皂甙元的合成。It was reported in the paper that the effect of different hormones on callus growth and diosgenin synthesis in callus of Dioscorea zingiberensis C.H.Wright.For the callus growth,different auxins' effect were in order of NAA,IAA,2,4\|D(NAA>IAA>2,4\|D).Their effect on diosgenin synthesis were just converse with foregoing states.Low concentration auxins promoted synthesizing of diosgenin.With auxin existing,0.5mg/L of BA and KT promoted the callus growth when the callus was exposed to light for 12 and 24 hours respectively Oppositely,0.5mg/L of BA and KT promoted diosgenin synthesis when the callus was exposed to light for 24 and 12 hours respectively.In general,The more exuberant the callus grew,the less the diosgenin synthesized.\;

关 键 词：激素 盾叶薯蓣 愈伤组织 细胞生长 薯蓣皂甙元 合成 调控 

分 类 号：S632[农业科学—蔬菜学]