pH值对转基因鱼腥藻培养的影响  

Effect of pH on the culture of recombinant Anabaena sp-PCC7120

作　　者：张晨[1] 刘志伟[1] 郭勇[2] 

机构地区：[1]嘉应学院生物系,广东梅州514015 [2]华南理工大学食品与生物工程学院,广东广州510640

出　　处：《生物技术》2002年第3期31-32,共2页Biotechnology

基　　金：广东省自然科学基金资助项目(980154)

摘　　要：研究pH值对转基因鱼腥藻培养的影响,发现培养基初始pH以自然pH8 3为宜。间歇流加稀盐酸控制过程pH值为8 5和9 0,生长较未流加快,每天流加稀盐酸一次控制过程pH为8 5,15d生物量大于3g/L,可溶性蛋白为0 3g/l藻干重,TNF表达量超过22%,生物量比未流加增长12 41%,而TNF表达量未受影响。Effect of pH was studied on the culture of recombinant Anabaena sp.PCC7120.The results showed that the optimal initial pH was 8.3.When pH was controlled to 8.5～9.0 by adding diluted HCl,the cell growth could be increased and the TNF expression could not be influenced.Under the optimal cultrue condition(pH was kept 8.5 during culture process),the biomass can exceed 3g/L,dissolvable protein was about 0.3g per gram dry weight cell,TNF expression level was more than 22%,the biomass increased 12.41% compared with cultured under nature pH.

关 键 词：PH值 转基因鱼腥藻 培养 肿瘤坏死因子 

分 类 号：TQ460.38[化学工程—制药化工] Q949.2[生物学—植物学]