证、病结合的子宫内膜异位症动物模型的复制  

Establishment of Animal Model of Endometriosis with TCM Symptoms of “Liver Depression”

作　　者：肖纯[1] 黄桂林[1] 刘小刚[1] 余跃飞 黄越燕 

机构地区：[1]江西中医学院,南昌330006 [2]浙江嘉兴医学院

出　　处：《中国实验动物学杂志》2002年第3期157-160,共4页Chinese Journal of Laboratory Animal Science

基　　金：江西省自然科学基金资助 (964 0 75 )

摘　　要：目的　复制具有中医“肝郁”表现的子宫内膜异位症大鼠模型 ,并进行病理观察。方法　采用手术移植法加用肾上腺素皮下注射法复制模型。取动情期大鼠子宫内膜片段移植于卵巢周围或腹壁内侧面。 3～ 4周后再以 0 1%肾上腺素 0 15ml 只 ,作皮下注射 ,每周一次 ,共 4次。 3周后 ,“肝郁”模型即成。对照组只作腹壁切开术 ,不作内膜移植。结果　移植物大体呈小囊状 ,色白透明或暗红 ,显微镜下小囊具有子宫内膜的基本组织结构 ,模型动物具有中医“肝郁”的临床症状及肾上腺的病理改变。结论　造型方法简便易行 ,周期短 ,成活率高 。Objective To establish and observe the rat model of endometriosis (EMT) with symptoms of Traditional Chinese Medicine (TCM). Methods The rat model of endometriosis was established by transplantation of endometrium and by hypodermatic injection of Adr method .The fragment of endometrium of estrous rat was transplanted around ovary or in abdominal wall.Three to four weeks later,0.1% Adr was added to it by hypodermatic injection(per rat was given 0 15ml),once a week,totally four times.Three weeks later,the rat model of 'liver depression' was established.The control group was only done by the operation of cutting abdominal wall open without endometrium transplantation.Results The transplantation object was of cyst shape and the colour of cyst was white and hyaline or dim red .The cyst had the structure of endometrium when it was observed with microscope.The model had TCM symptoms of 'liver depression' and there were some pathological changes in the tissue of adrenal.Conclusion The model was established simply and easily with a high success rate .It could contribute to the study of TCM.

关 键 词：病理 证病结合 子宫内膜异位症 动物模型 复制 

分 类 号：R271.19[医药卫生—中医妇科学] R－332[医药卫生—中医学]