水稻胚乳细胞质膜内陷的超微结构和磷酸酶的细胞化学定位  

The Ultrastructure and Cytochemical Localization of Phosphatase Activity of Plasmalemma Invagination in Endosperm Cells of Rice

作　　者：韦存虚[1] 蓝盛银[1] 徐珍秀[1] 

机构地区：[1]华中农业大学生命科学技术学院,武汉430070

出　　处：《植物生理与分子生物学学报》2002年第3期221-226,共6页Journal Of Plant Physiology and Molecular Biology

基　　金：国家自然科学基金 ( 30 0 70 36 3);高等学校博士学科点专项科研基金 ( 2 0 0 0 0 5 0 40 1)资助项目

摘　　要：对生长分化期水稻胚乳细胞的质膜内陷进行了超微结构和磷酸酶的细胞化学研究。结果表明 ,胚乳细胞内的小泡、内质网常与胞间连丝相连 ;质膜形态多变 ,功能活跃 ,由局部起伏的波纹状发展成明显内陷 ,深浅不一 ,多呈袋状 ,袋中包含着大小不一的泡状物 ;有些内陷脱离质膜成为胞质中的囊泡 ,表现出活跃的内吞现象。除细胞间隙中含有圆球状的内含物外 ,在质膜内陷和囊泡中常含有大量的内含物。H+ ATP酶定位结果显示 ,质膜及其邻近的泡状物周围有酶的分布 ;而酸性磷酸酶定位在液泡、胞间隙和其中的泡状内含物周围 ;在质膜及其内陷形成的囊泡中有G6P酶的分布。The plasmalemma in endosperm cells of rice showed invagination with developing and differentiating. The ultrastructure and phosphatase activity cytochemistry of these invaginations were studied. Some vesicles and many endoplasmic reticulum elements were associated with the plasmodesmata (Figs.2, 3, 5, 6). Plasmalemma appeared undulated, but occasionally pocketable and variable in size with conspicuous invaginations (Figs.4-8). Some invaginations were separated from plasmalemma to form sacs in the peripheral cytoplasm (Fig.9). Some spherical inclusions appeared in the intercellular space (Fig.3), the plasmalemma invaginations (Figs.4-8) and the sacs (Fig.9). H +-ATPase localization showed high enzymic activity in the plasmalemma (Fig.10) and vesicles beside the plasmalemma (Fig.11). Activity of acid phosphatase (AcPase) was observed in the vacuole, the intercellular space and its vesicular contents (Figs.12, 13). The plasmalemma and sacs in the peripheral cytoplasm exhibited high activities of glucose-6-phosphatase (G6Pase) (Figs.14-16). These results suggested that the intercellular space and plasmalemma invagination could played an important role in transportation of nutrients.

关 键 词：水稻 胚乳细胞质膜内陷 超微结构 磷酸酶 细胞化学定位 

分 类 号：S511.01[农业科学—作物学]