用噬菌体随机肽库鉴定日本血吸虫22.6kDa抗原分子的表位  

Identification of Epitope of 22.6 kDa Antigen of Schistosoma japonicum with Phage-displayed Random Peptide Library

作　　者：胡雪梅[1] 张兆松[2] 吴海玮[2] 苏川[2] 季旻珺[2] 王勇[2] 陈淑贞[2] 吴观陵[2] 

机构地区：[1]滨州医学院免疫学教研室,滨州256603 [2]南京医科大学分子生物学研究所,南京210029

出　　处：《中国寄生虫学与寄生虫病杂志》2002年第3期164-167,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：总理预备金血防专项基金 (No.94 Y 2 3)资助;国家自然科学基金 (No .3980 0 316 )部分资助~~

摘　　要：目的　筛选和鉴定日本血吸虫 (中国大陆株 ) 2 2 .6kDa抗原 (Sj2 2 .6)的表位。　 方法　用纯化的抗Sj2 2 .6的多克隆抗体IgG对噬菌体十二肽库进行 5轮免疫学筛选 ,挑取克隆 ;采用Westernblotting免疫识别 ,将获得的阳性克隆免疫小鼠 ,并采用dot ELISA筛选能刺激小鼠产生较高滴度抗Sj2 2 .6抗体的阳性克隆 ;测定核苷酸序列 ,分析其表位与Sj2 2 .6抗原的同源性。　结果　经 5轮免疫学筛选后挑取的 1 4个克隆 ,用Westernblotting方法均能被抗Sj2 2 .6抗体识别。经动物免疫初步实验筛选 ,共获得 6个免疫原性较强的阳性克隆 ,测序结果获得 4种不同表位 ,其中 1种表位与Sj2 2 .6抗原具有较高的同源性 ,其余 3种表位与其无一级结构的同源性。　结论　获得的 4种日本血吸虫中国大陆株抗原表位 ,1种可能为结构表位 。Objective To identify the epitopes of 22.6 kDa antigen of Schistosoma japonicum (Sj22.6). Methods A 12 mer library displayed on pIII of fd phage was used to screen the epitopes of Sj22.6 with the purified multiple clone IgG antibody against the Sj22.6 antigen. Five rounds of biopanning were carried out and fourteen clones from the fifth round biopanning were randomly selected and identified by Western blotting. The mice were immunized with the obtained positive clones and the antibody titers of sera from the mice were detected. The clones which could stimulate the mice to produce anti Sj22.6 antibodies were sequenced and their amino acid sequences were compared with that of Sj22.6. Results Six clones selected from the fourteen ones could stimulate the mice to produce anti Sj22.6 antibodies analysed by Western blotting. The amino acid sequence of one epitope showed high homology with Sj22.6. Conclusion Four epitopes of Sj22.6 were obtained. One may be a structure epitope and the other three were mimic epitopes.

关 键 词：22.6kDa抗原 噬菌体肽库 表位 日本血吸虫 Sj22.6抗原 血吸虫疫苗 

分 类 号：R532.21[医药卫生—内科学]