Cu,Zn-SOD在银电极上的原位表面增强Raman光谱  

In-situ Surface Enhancement Raman Spectra of Cu,Zn -Superoxide Dismutase on Silver electrode

作　　者：杨海峰[1] 曹晓卫[1] 王桂华[1] 章宗穰[1] 

机构地区：[1]上海师范大学化学系,上海200234

出　　处：《光散射学报》2002年第2期106-110,共5页The Journal of Light Scattering

基　　金：上海市科技启明星计划资助项目 (0 0QA14 0 17)

摘　　要：本文应用原位共焦拉曼光谱技术观察了Cu ,Zn -SOD分子在经表面增强处理后的银电极上 ,吸附状态随电位跃迁变化的过程。结果表明在 -0 2V～ 0 1V的电位区间内 ,Cu,Zn -SOD分子经由羧酸根离子吸附于银电极表面 ,而在 0 2～ 0 4V电位区间内发生脱附 ,表面吸附的物种改变为缓冲溶液中的磷酸根离子。上述结果为SOD分子在固体电极表面电子传递机制的探索提供了依据。Adsorption of Cu,Zn -Superoxide Dismutase (Cu,Zn-SOD) on pretreated silver electrode has been studied by in-situ Raman spectroelectrochemical method It was observed that the adsorption of Cu,Zn-SOD was changed with the applied potential The Cu,Zn-SOD molecules were adsorbed onto the surface of silver electrode via COO - group at the potential region from -0 2 to 0 1V, but it was desorbed from the electrode surface from 0 2～0 4V mostly and was replaced by the adsorption of the phosphate ions from the buffer solution The observed SERS spectra is consistent with the predicted electron transfer process of mentioned SOD system with gold electrode

关 键 词：CU ZN-SOD 超氧化物歧化酶 SOD 表面增强拉曼光谱 铜 锌 生物催化剂 原位Raman光谱 电化学 银电极 

分 类 号：O646.5[理学—物理化学] Q554.9[理学—化学]