延时分幅扫描单光子计数荧光光谱技术  

DELAY PAPTING FRAME SCANNING SINGLE PHOTON COUNTING FLUORESCENCE SPECTROSCOPY

作　　者：贺俊芳[1,2] 王水才[1] 任兆玉[1,2] 蔡霞[1,2] 

机构地区：[1]中国科学院西安光学精密机械研究所瞬态光学技术国家重点实验室 [2]西北大学,西安710069

出　　处：《光子学报》2002年第7期807-810,共4页Acta Photonica Sinica

基　　金：国家重点基础研究专项经费 (G1 9980 1 0 1 0 0 );所长基金资助项目

摘　　要：设计建立了一套延时分幅扫描单光子计数荧光光谱装置 ,以雪崩光电二极管 SPCM-AQR- 1 5为荧光探测器 ,T91 4P单光子计数模件为信号接收仪器 ,通过对 T91 4P的触发信号加延时 ,在每个延时下对荧光信号进行分幅扫描 ,再将所有延时的分幅扫描信号叠加在一起 ,可以将快速的微弱信号很好地再现出来 ,时间分辨率提高了 1 6.7倍 ,采用此技术对光合作用CP43色素蛋白复合物进行了研究 ,荧光光谱解叠可分辨出三个窄谱带 :681 nm、684nm、72 9nm,并分别在 670 nm,680 nm和 685 nm荧光发射处 ,采用全局优化拟合法处理 ,获取了 3个寿命组分 :42 2 ps,5 82 ps,3.75 ns,其中 42 2 ps反应了 Chla671到 Chla679和 Chla682能量传递过程 ;5 82 ps代表 Chla679到 Chla682的能量传递 ;3.75 ns代表The measurement of ultrafast faint light signal has always been a focus of ultrafast spectral research.A setup of delay parting frame scanning single photon counting fluorescence spectrum were designed and founded. Fluorescence emission spectrum were measured with a avalanche photoelectric diode SPCM AQR 15 and single photon counting module T914P. To restore finely the fast and faint signal, time delays were added in the trigger of T914P.At each delay the fluoresence signal was scanned and added together. The technology could exert mostly the superior capability of fast photoelectric detector, and improve the time resolution to 16.7 times. Photosynthesis CP43 pigment protein complex were studied. The fluorescence spectrum and three gauss decomposition bands 681nm?684nm?729nm of spectrum were gotten. With global optimization way , three lifetime components were necessary for a good fit of the functions of fluorescence intensity at 670nm, 680nm, 685nm and time. The shortest lifetime of 422ps, was attributed to energy transfer from Chla671 to Chla679 and Chla682. The lifetime of 582ps was attributed to energy transfer from Chla679 to Chla682. The longest lifetime of 3.75ns was attributed to the sum of all deexcitation rate of Chla 682.

关 键 词：延时分幅扫描 荧光光谱 单光子计数 光系统Ⅱ CP43 叶绿素 光电探测系统 生物发光 

分 类 号：Q945.11[生物学—植物学] TN2[电子电信—物理电子学]