ELSD-HPLC法测定浙麦冬、川麦冬中麦冬皂苷D含量的方法研究  

Quantitative determination of Ophiopogonin D in Zhe Ophiopogon japonicus(Thunb.)Ker-Gawl. and Chuan Ophiopogon japonicus(Thunb.) Ker-Gawl. by HPLC-ELSD

作　　者：俞建平[1,2,3] 马月光[1,2,3] 邵建峰 祝明[1,2,3] 

机构地区：[1]浙江省药品检验所,杭州310004 [2]杭州市第一人民医院,杭州310005 [3]正大青春宝药业有限公司,杭州310011

出　　处：《中药新药与临床药理》2002年第4期253-255,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家药品监督管理局药典委员会资助课题

摘　　要：目的采用蒸发光散射检测器(ELSD)测定浙麦冬、川麦冬中麦冬皂苷D的含量。方法色谱条件:AlltimaC18柱(250mm×4.6mm,5mm);漂移管温度98℃,气体流量2.8L/min;流动相为乙腈-水(55∶45);流速:1.0mL/min,柱温30℃。结果与结论麦冬皂苷D在0.2565～6.4125μg范围内呈良好的线性关系,r=0.9992;平均加样回收率为102.0%,RSD=2.1%(N=6)。HPLC-ELSD was used to determine the content of Ophiopogonin D in Zhe Opiopogon japonicus(Thunb.) Ker-Gawl. and Chuan Ophiopogon japonicus(Thunb.) Ker-Gawl. The chromatographic conditions were as follow: Alltima C18 column (250mm×4.6mm) and the column temperature at 30℃, drift tube temperature at 98 ℃with Gas flow rate being 2.8 L/min and acetonitrile : water (55∶45) as mobile phase with the flow rate being 1.0 mL/min. There was a good linearity (r=0.9992) within the range of 0.2565～6.4125 μg. The average recovery rate was 102.0%and RSD=2.1%.

关 键 词：麦冬 麦冬皂苷D 中药产地 ELSD-HPLC 

分 类 号：R284.1[医药卫生—中药学]