斑点金免疫渗滤法检测不孕症患者血清抗子宫内膜抗体  

Dot-immunogold filtration assay for detection of antiendometrial antibody in serum of infertile couples

作　　者：许国强[1] 杜耀武[1] 马远方[1] 张军[1] 白慧玲[1] 赵粤萍[1] 

机构地区：[1]河南大学生殖医学中心,开封475001

出　　处：《郑州大学学报（医学版）》2002年第4期452-454,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：河南教育厅自然科学基金资助项目 95 199

摘　　要：目的 :建立一种快速测定抗子宫内膜抗体 (antiendometrialantibody ,EMAb)的新方法。 方法 :采用经抗人血清抗体 Sepharose4B免疫吸附柱亲和层析纯化的人子宫内膜抗原 (endometriumantigen ,EMAg) ,以胶体金颗粒结合的羊抗人IgG为标记抗体 ,根据免疫渗滤原理 ,建立斑点金免疫渗滤法 (Dot immunogoldfiltrationassay ,DIG FA)检测人血清EMAb。共检测 5 86份不孕者血清并与酶联免疫吸附试验 (ELISA)作对比研究。结果 :纯化的人子宫内膜抗原与患者血清中特异性EMAb通过渗滤在硝酸纤维素膜上反应 ,8min内即可直接观察结果。在 5 86份血清的检测中 ,阳性率为 36 .8%,与ELISA的结果基本一致 (χ2 =3.45 ,P >0 .0 5 ) ,符合率 95 .0 %。特异性为94.7%,敏感性为 95 .6 %。交叉试验与重复试验结果显示DIGFA具有较好的特异性及稳定性。结论 :DIGFA可快速测定EMAb ,具有推广使用的价值。Aim: To study a new method to identify antiendometrial antibody quickly in serum. Methods: Purifying endometrium antigen with antiserum-Sepharose4B chemical solutions,then combining it with the nitrocellulose membrane,and taking goat anti-IgG of human combined with the golden pellets of colloidal state as marked antibody to examine antiendometrial antibody via the dot-immunogold filtration assay (DIGFA).A total of 586 clinical serum samples were examined by this method and the results were compared with those detected by ELISA. Results: The antiendometrial antibody and endometrium antigen reacted on the membrane by filtration and the result could be observed with naked eyes within 8 min.The examination of 586 clinical serum samples showed that the positive rate was 36.8%.The result of this method was in accordance with that of ELISA(χ 2=3.45,P>0.05).The specificity and sensitivity were 94.7% and 95.6%. Cross test and repeated test showed that DIGFA had better specificity and steadiness. Conclusion: The dot-immunogold filtration assay can examine antiendometrial antibody quikly and is worth spreading.

关 键 词：斑点金免疫渗滤试验 酶联免疫吸附试验 抗子宫膜抗体 不孕症 

分 类 号：R711.6[医药卫生—妇产科学]