急性胰腺炎胰腺血液流变学改变与细胞粘附分子的表达  

Hemorheology and expression of neutrophil adhesion molecules CD18 and CD62L in pancreatic microcirculation of Caerulein induced experimental acute pancreatitis

作　　者：周总光[1] 陈友琴[2] 于永杨[1] 陈槐卿[2] 

机构地区：[1]四川大学华西医院普外科,成都610041 [2]四川大学华西医学中心生物医学工程研究室

出　　处：《中华外科杂志》2002年第7期528-530,共3页Chinese Journal of Surgery

基　　金：国家自然科学基金及国家杰出青年基金资助项目(3 9770 72 2 ;3 992 5 0 3 2 )

摘　　要：目的　探索急性胰腺炎 (AP)实验动物模型的早期胰腺局部血液流变学改变、流体切应力作用下中性粒细胞表面粘附分子表达在AP发展中的作用。　方法　将 2 1只Wistar大鼠随机分为3组 ,用蛙皮缩胆囊肽 (caeralein)诱发AP动物模型 ,用Low shear 30血流流变仪检测全血表观粘度 ,用流式细胞仪分析切应力介导的中性粒细胞表面粘附分子的表达。　结果　 (1)各实验组血清淀粉酶升高 ,与正常组相比 ,差异有显著性 (t=6 9 0 2 9,t =79 734,P <0 0 5 )。 (2 )不同切变率下各实验组的全血表观粘度均增高 ,尤以低切变率下增高更为显著 (0 5 12s-1:t=10 72 5 ,t=16 94 5 ;5 96s-1:t=12 781,t=11 992 ,P <0 0 5 )。 (3)在切变率作用下各组CD18的表达随着切变率的升高而增加 ,且在切变率为 94 5s-1和 12 8 5s-1时 ,有显著性差异 (94 5s-1:t=7 4 0 3,t=13 32 3,t=16 6 5 5 ;12 8 5s-1:t=10 0 92 ,t=2 8 5 31,t=2 4 5 6 3,P <0 0 5 )。 (4)低切变率作用对不同组别CD 6 2L的表达无显著影响 ,当切变率升高≥ 94 5s-1时 ,各组CD 6 2L的表达开始下调 ,差异有显著性 (94 5s-1:t=10 6 87,t=19 376 ,t=12 84 8;12 8 5s-1:t=2 6 15 2 ,t=4 8 4 0 2 ,t=5 6 814 ,P <0 0 5 )。　结论　AP早期胰腺微循环全血表观粘?Objective To explore the changes in hemorheology and expression of neutrophil adhesion molecules CD18 and CD62L in pancreatic microcirculation of Caerulein induced experimental acute pancreatitis (AP). Methods The Wistar rats ( n =21) were randomized into three groups. The model of AP was established by subcutaneous injection of Caerulein. The changes of apparent viscosity of whole blood were measured by Low shear 30 rheometer. The expression of adhesion molecules on the surface of neutrophil in duced by shear stress was used with stationary control. CD18 expression was increased on neutrophils treated with shear rate, and andanalyzed using flow cytometry. Results Rat treated with Caerulein showed hyperamyleimia ( t =69 029, t =79 734 , P <0 05). Blood viscosity of two AP groups were significantly elevated (0 512 s -1 : t =10 725, t =16 945; 5 96 s -1 : t =12 781, t =11 992, P <0 05). Compared with stationary control,CD18 expression was increased on neutrophil treated with shear rate, and significantly induced with shear rate ≥94 5 s -1 (94 5 s -1 : t =7 403, t =13 323, t =16 655; 128 5 s -1 : t =10 092, t =28 531, t =24 563, P <0 05). The expression of CD62L was less sensitive to low shear rate, and began to be down regulated significantly when the shear rate≥94 5 s -1 (94 5 s -1 : t =10 687, t =19 376, t =12 848;128 5 s -1 : t =26 152, t =48 402, t =56 814, P <0 05). Conclusions The changes of apparent viscosity of whole blood, and the effect of fluid shear stress on the expression of neutrophil adhesion molecules CD18,CD62L may play an important role in the pancreatic microcirculatory failure of acute pancreatitis.

关 键 词：急性胰腺炎 胰腺 血液流变学 细胞粘附分子 流体切应力 

分 类 号：R657.51[医药卫生—外科学]