骨关节炎基质金属蛋白酶1、2、3及其抑制物的表达与软骨退变的关系  

Immunohistochemical Expression of Matrix Metalloproteinase-1,2,3 and Their Tissue Inhibitors in Osteoarthritis:Its Relationship with Cartilage Degeneration

作　　者：郭常安[1] 陈峥嵘[1] 张秀荣[2] 

机构地区：[1]复旦大学附属中山医院骨科,上海200032 [2]复旦大学医学院病理学教研室,上海200032

出　　处：《复旦学报（医学版）》2002年第4期244-246,F003,共4页Fudan University Journal of Medical Sciences

基　　金：卫生部科研基金资助 ( 98-2 -132 )

摘　　要：目的　探讨骨关节炎关节软骨中基质金属蛋白酶 (MMPs) 1、2、3及其抑制物 (TIMPs) 1、2的免疫组化表达及与软骨退变的关系。方法　 2 2例因骨关节炎行关节置换的软骨组织常规HE染色观察其组织学变化 ,ABC免疫组化法观察关节软骨MMP 1、2、3和TIMP 1、2的表达 ,经图像分析研究MMP 1、2、3和TIMP 1、2与软骨组织学退变的关系 ,5例因股骨颈骨折行关节置换的软骨标本为正常对照。结果　骨关节炎关节软骨出现纤维化、裂隙、变薄 ,软骨细胞数量减少 ;MMP 1、3和TIMP 1在正常软骨无表达 ,在中度退变软骨中表达明显升高 ,在重度退变软骨中表达降低 ,TIMP 1与MMP 1表达升高不平行 ;MMP 2和TIMP 2在正常软骨低表达 ,在中度退变软骨中表达升高 ,在重度退变软骨表达降低 ,但TIMP 2表达升高。结论　在骨关节炎中MMP 1、2、3和TIMP 1、2表达异常 ,引起细胞外基质异常降解 。Purpose. To investigate the relationship between immunohistochemical expression of matrix metalloproteinase(MMPs)-1,2,3/their tissue inhibitor of metalloproteinase (TIMPs)-1,2 and the degeneration of cartilage in osteoarthritis. Methods. The histological changes of degenerated cartilages by haematoxyllin-eosin staining and the immunohistochemical expressions of MMPs-1,2,3 and TIMPs-1,2 were studied in 22 osteoarthritis cases. The image analysis revealed the relationship between the expressions of MMP-1,2,3, TIMP-1,2 and the degeneration of cartilage. Results. The osteoarthritis cartilage underwent fibroplasia, tearing and thinning. The quantity of chondrocyte decreased. The expressions of MMP-1,3 and TIMP-1 were not observed in normal cartilage, while increased significantly in moderate osteoarthritis and decreased in end-stage osteoarthritis. The expression of TIMP-1 was not paralleled with that of MMP-1,3. Little immunostaining of MMP-2 and TIMP-2 was seen in normal cartilage, while their expressions increased in moderate osteoarthritis and decreased in end-stage osteoarthritis. In end-stage osteoarthritis, the expressions of TIMP-2 was stronger than that of MMP-2. Conclusions. The abnormal expressions of MMP-1,2,3 and TIMP-1,2, which resulted in degradation disorder of extracellular matrix, could induce degeneration of cartilage.

关 键 词：骨关节炎 基质金属蛋白酶 基质金属蛋白酶组织抑制因子 免疫组织化学 

分 类 号：R684.3[医药卫生—骨科学]