利多卡因、异丙酚对缺血再灌注心肌细胞膜Na^+-K^+-ATP酶、肌浆网Ca^(2+)-ATP酶活性的影响  

Effects of lidocaine and propofol on the activities of myocardial sarcolemmal Na^+-K^+-ATP ase and sarcoplasic reticulum Ca^(2+)-ATPase following ischemia-reperfusion

作　　者：刘红亮[1] 戴体俊[1] 王钧[1] 曾因明[1] 

机构地区：[1]徐州医学院麻醉医学研究所,徐州221002

出　　处：《中国临床药理学与治疗学》1999年第2期140-142,共3页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：卫生部科研基金资助课题(NoH9602)

摘　　要：目的观察利多卡因、异丙酚对缺血再灌注心肌细胞膜Na+ _K+ _ATP酶、肌浆网Ca2 +_ATP酶活性的影响。方法SD大鼠48只 ,随机均分为6组。假手术组 ;生理盐水组 ;利多卡因5mg·kg-1 组 ;利多卡因10mg·kg-1 组 ;异丙酚300μg·kg-1·min-1 组 ;异丙酚1000μg·kg-1·min-1 组。于缺血前5min ,生理盐水及利多卡因经股静脉注射给药(30s内注射完) ;异丙酚持续输注至缺血开始时为止。结扎冠脉左前降支 ,造成左室前壁相应心肌组织缺血15min ,然后松开结扎线再灌注10min ,应用分光光度法测定心肌细胞膜Na+_K+ _ATP酶、肌浆网Ca2 + _ATP酶活性。结果利多卡因可明显地促进肌浆网Ca2 + _ATP酶活性的恢复(P<0.05) ,大剂量时还促进心肌细胞膜Na+ _K+ _ATP酶活性的恢复(P<0.05)。异丙酚可显著地促进心肌细胞膜Na+ _K+ _ATP酶活性的恢复(P<0.05 ,P<0.001),大剂量时促进肌浆网Ca2 + _ATP酶活性的恢复(P<0.05)。结论利多卡因、异丙酚可促进再灌注期间心肌细胞膜Na+ _K+ _ATP和肌浆网Ca2 + _ATP酶活性的恢复 ,从而抑制钙超载而减轻心肌缺血再灌注损伤。Aim To investigate the effects of lidocaine and propofol on the activities of myocardial sarcolemmal Na+- K+-ATPase and SR Ca2+-ATPase following ischemia-reperfusion. Methods Forty-eight rats were randomly divided into six groups:Sham group(the coronary was not sutured),NS group (saline 5 ml·kg-1), L5 group (lidocaine 5 mg·kg -1),L10 group (lidocain 10 mg·kg-1),P300 group (propofol 300 μg·kg-1·min-1) and P1000 group (propofol 1000 μg·kg-1·min-1).Five minutes before ischemia, Saline or lidocaine was injected intraveneously within 30 seconds and propofol was infused intraveneously till the onset of ischemia. The left coronary artery was occluded with a silk suture for fifteen minutes, then loosened to achieve reperfusion for ten minutes. The activities of sarcolemmal Na+-K+-ATPase and SR Ca2+-ATPase were measured with photocolormetre.Results Compared with that in NS group the activities of SR Ca2+-ATPase in the lidocaine groups were increased significantly (P<0.05),while the activites of sarcolemmal Na+-K+-ATPase in propofol groups were increased significantly(P<0.05,P<0.01 respectively).Propofol of 1000 μg·kg-1·min-1 could increase the activity of SR Ca2+-ATPase (P<0.05). Lidocaine of 10 ml·kg-1 could increase the activity of sarcolemmal Na+-K+-ATP ase significantly(P<0.05) .Conclusion Lidocaine, propofol could attenuate myocardial ischemia-reperfusion injury by increasing the activities of sarcolemmal Na+-K+-ATPase and SR Ca2+-ATPase during reperfusion.

关 键 词：缺血再灌注 心肌细胞膜 利多卡因 异丙酚 NA^+-K^+-ATP酶 肌浆网Ca^2+-ATP 

分 类 号：R971.2[医药卫生—药品]