性外激素诱导大弹涂鱼成熟和产卵  

Induced maturation and spawning by pheromones in female mudskipper (Boleophthalmus pectinirostris)

作　　者：赵卫红[1] 洪万树[1] 吴鼎勋[1] 姜欣欣[1] 张其永[1] 

机构地区：[1]厦门大学海洋系,福建厦门361005

出　　处：《台湾海峡》2002年第3期284-291,共8页Journal of Oceanography In Taiwan Strait

基　　金：国家自然科学基金资助项目 (30 170 739) ;福建省自然科学基金资助项目 (B0 0 10 0 0 5 )

摘　　要：成熟大弹涂鱼的卵巢、精巢和储精囊稀释液中的性外激素以及缩宫素和脱氧皮质酮 (DOC)能诱导第 4时相的大弹涂鱼卵母细胞在离体培养的条件下发育成熟 ,成熟率与性外激素的种类和剂量有关 .前列腺素E1(PGE1)单独使用的效果不明显 ,但与人绒毛膜促性腺激素 (HCG)混合使用时显著提高了卵母细胞的成熟率 .以卵巢、精巢和储精囊稀释液以及PGE1分别注射雌性大弹涂鱼 ,剂量为 0 .0 1cm3/尾的卵巢液、0 .1cm3/尾的储精囊液、0 .1cm3/尾的精巢液以及剂量为 1 0 μg/尾的PGE1都有诱导卵母细胞成熟的作用 ,其中储精囊液的作用最为显著 .在人工管道中放置大弹涂鱼成熟亲鱼卵巢、精巢或储精囊的稀释液 ,有诱导异性亲鱼进入管道内的作用 ,其中以储精囊液对雌鱼的吸引作用尤为明显 .放置于陶瓷管道或海绵管道内的卵巢、精巢或储精囊稀释液都能诱导大弹涂鱼产卵 ,在陶瓷管道的产卵量和受精率显著高于海绵管道 .Maturation of the 4th phase oocytes of mudskipper was induced in vitro by pheromones in the extract of the ovary, testis and seminal vesicle,as well as oxytocini and deoxycorticosterone (DOC). Maturation rates of oocytes related to not only pheromones but also their dosa- ges. The single use of PGE 1 was not effective in induction of maturation, but the combination of prostaglandin E 1(PGE 1) with HCG had a coordinated effect in improving ovulation. Maturation of female mudskippers with the 4th stage of ovary were effectively induced by injections of the extract of the ovary at dosages of 0.01cm 3/a fish and 0.1cm 3/a fish, seminal vesicle at dosages of 0.01cm 3 /a fish and 0.1cm 3/a fish, testis at dosage of 0.1cm 3/a fish and PGE 1 at dosage of 10μg/a fish. Among the three gonadal extract, seminal vesicle one was the most effective in the induction of maturation. Compared with the controls, those artificial nests, which had the extract of the ovary, testis and seminal vesicle inside, attracted more fish to enter into. The glands' extract attracted more heterosexual mudskippers than homosexual ones to enter the nests. Especially, the extract of seminal vesicle was more effective in attracting heterosexual individuals than ovary and testis. Female mudskippers could be induced to spawn or attracted to enter nests by the extract of glands set inside the ceramic nests. Time of spawning, the number of spawned eggs and the fertilizing rates of matured eggs were related to the types of the nests.

关 键 词：大弹涂鱼 性外激素 成熟 产卵 卵巢 精巢 缩宫素 

分 类 号：S965.299[农业科学—水产养殖] Q959.483[农业科学—水产科学]