A Broad Spectrum Identification Approach for Two Generations of epsps GM Soybeans  

作　　者：Qiuchi SONG Xiwei LIU Yiwei MOU Yuan JING Jun SONG 

机构地区：[1]Class 10 Grade 2018,Wenmiao School of Shishi High School [2]Teaching and Research Group of Biology,Wenmiao School of Shishi High School [3]Quality and Safety Center of Agricultural Products of Ganzi Prefecture of Sichuan Province [4]Analysis and Determination Center,Sichuan Academy of Agricultural Sciences

出　　处：《Asian Agricultural Research》2017年第12期59-63,共5页亚洲农业研究（英文）

基　　金：Supported by Project for Promotion of Financial Innovation Ability of Sichuan Province(2016GXTZ-010);Dongrun-Yau Science Award(Biology,2016)

摘　　要：In order to accurately identify the first and second generations of epsps genetically modified(GM) soybeans and related products,a broad spectrum identification approach was established using the real time Polymerase Chain Reaction(PCR) principle according to the homology of epsps genes of the first and second generations of GM soybeans.A pair of primer and probe was designed to simultaneously identify exogenous gene epsps of two generations of GM soybeans.Besides,evaluation was carried out on this approach from the accuracy,specificity,sensitivity and reproducibility.The experimental results indicated that(ⅰ) although there is certain difference in epsps gene sequence between the first and second generations of epsps genetically modified(GM) soybeans,the established approach can simultaneously detect the epsps genes of the bean curd using two generations of soybean as raw materials;(ⅱ) in the accuracy and specificity experiment,only cp4-epsps genes of two generations of GM soybeans were detected,so this approach has high specificity and accuracy;(ⅲ) in the experiment of 5 copies of epsps genes of 40 repeated identification reaction systems,5 copies of epsps genes can be detected each time,therefore at 100% confidence level,this approach can identify 5 copies of epsps genes,showing that this approach has high sensitivity and reproducibility.In order to accurately identify the first and second generations of epsps genetically modified(GM) soybeans and related products,a broad spectrum identification approach was established using the real time Polymerase Chain Reaction(PCR) principle according to the homology of epsps genes of the first and second generations of GM soybeans.A pair of primer and probe was designed to simultaneously identify exogenous gene epsps of two generations of GM soybeans.Besides,evaluation was carried out on this approach from the accuracy,specificity,sensitivity and reproducibility.The experimental results indicated that(ⅰ) although there is certain difference in epsps gene sequence between the first and second generations of epsps genetically modified(GM) soybeans,the established approach can simultaneously detect the epsps genes of the bean curd using two generations of soybean as raw materials;(ⅱ) in the accuracy and specificity experiment,only cp4-epsps genes of two generations of GM soybeans were detected,so this approach has high specificity and accuracy;(ⅲ) in the experiment of 5 copies of epsps genes of 40 repeated identification reaction systems,5 copies of epsps genes can be detected each time,therefore at 100% confidence level,this approach can identify 5 copies of epsps genes,showing that this approach has high sensitivity and reproducibility.

关 键 词：Genetically modified(GM) soybeans epsps gene Broad spectrum SPECIFICITY Sensitivity 

分 类 号：S565.1[农业科学—作物学]