原核注射法转基因猪外源基因整合位点的分布及其遗传规律  

Distribution and Genetical Characteristics of Sites Combined Foreign Gene in Transgenic Pig Produced by Prenuclear Microinjection

作　　者：魏庆信[1] 樊俊华[1] 郑新民[1] 华文君[1] 陈清轩[2] 李奎[3] 陈付学[4] 

机构地区：[1]湖北省农业科学院生物技术研究所,湖北武汉430064 [2]中国科学院发育生物学研究所,北京100080 [3]华中农业大学畜牧兽医学院,湖北武汉430070 [4]上海大学生命科学院,上海200436

出　　处：《中国兽医学报》2002年第4期390-391,共2页Chinese Journal of Veterinary Science

基　　金：国家"8 63"计划资助项目 (10 1-0 5 -0 5 -0 1;10 1-0 5 -0 1-0 1)

摘　　要：应用非同位素标记染色体原位杂交技术 ,对 9头不同世代的转 OMT- PGH基因猪和 6头原代转 h DAF基因猪外源基因整合位点的分布及其遗传规律进行了研究。结果表明 ,4头原代转 OMT- PGH基因猪外源基因分别整合在第7、13、2和 8号染色体上 ,6头原代转 h DAF基因猪外源基因分别整合在第 13、6、11、2、7和 7号染色体上 ,证实了用原核注射法转基因猪外源基因的整合是“随机”的。来自同一亲本、不同世代的 5头转 OMT- PGH基因猪 ,其外源基因均存在于第 13号染色体上 ,与共同祖先原代转基因猪的整合位点相一致 ,表明转基因猪外源基因可以传递给后代 。Using non radio label chromosome situs blot technique,the distribution and heredity regulation of sites combined foreign gene in 6 finders with hDAF gene and 9 transgenic pigs from different generations with OMT PGH gene were investigated.The results indicated the OMT PGH gene located in chromosome 7,13,2 and 8 of 4 finder transgenic pigs.The hDAF gene sited in chromosome 13,6,11,2,7 and 7.This means that the foreign gene integration on chromosome is random when transgenic pig is produced by microinjection procedure.Five OMT PGH transgenic pigs from different generation shared the same integration site in chromosome 13 with their mutual ancester.This shows that the foreign gene may be heridable and its sits is stable.

关 键 词：原核注射法 外源基因 分布 遗传规律 原位杂交 染色体 整合位点 转基因猪 

分 类 号：S814.8[农业科学—畜牧学]