优良浆意蜂DNA分子特异标记(W316bp)的鉴定研究  

Study on Identification of special marker in DNA molecule of High royal jelly bee Api mellifera

作　　者：蒋滢[1] 张雅娟[1] 蒋菊香[1] 薛运波[2] 戴英[3] 徐颖[3] 

机构地区：[1]苏州大学医学院,江苏苏州215007 [2]吉林省养蜂研究所,吉林吉林132108 [3]苏州大学生命科学院,江苏苏州215007

出　　处：《蜜蜂杂志》2002年第8期3-4,共2页Journal of Bee

摘　　要：第一部分分离提取三品系意蜂 (浙农大、萧山、平湖 )的DNA ,使用紫外分光光度计 ,波长 2 6 0nm鉴定DNA纯度 ;第二部分以W (5′ -CGGCCCCGGT - 3′)为随机引物进行PCR ,扩增 ,电泳 ,获得DNA多态性图谱 ;第三部分比较三品系意蜂工蜂与雄蜂的DNA多态性图谱。结果凡是三品系意蜂的工蜂均出现一高产浆特异标记W316pb条带 ,但三品系意蜂的雄蜂均无W316bp条带。This study was divided into three parts,part one,DNA extracted from 3 race worker,male bees of high royal jelly Apis mellifera .Its purity treated of through UV Anlaysis,260nm; part two W random primer (5-CGGCCCCGGT-3) and PCR were used to amplify on a genetic map; three part worker bee compared with male bee in 3 race of high royal jelly Apis mellifera . Genetic map of worker bee appeared W316bp fragment,but male bee does not. It is important that worker bee of high royal jelly Apis mellifera ,only have W316bp fragment in genetic map.

关 键 词：DNA分子特异标记 鉴定 意蜂 PCR 高产浆蜂 

分 类 号：S891.1[农业科学—特种经济动物饲养]