实验性血管痉挛中内皮一氧化氮合酶的基因表达  

Endothelial NO synthase gene expression in experimental vasospasm in rabbits

作　　者：傅强[1] 傅向阳[1] 傅晓华[1] 姜立[2] 

机构地区：[1]第一军医大学珠江医院心内科,广东广州510282 [2]分子生物学研究所,广东广州510515

出　　处：《第一军医大学学报》2002年第7期629-631,共3页Journal of First Military Medical University

摘　　要：目的　研究内皮 eNOS基因表达与兔实验性血管痉挛的关系。方法　将16只兔随机分成内皮剥脱+普通饮食组（n=8）和内皮剥脱+胆固醇饮食组（n=8）。血管造影观察球囊内皮剥脱前后以及普通饮食和高胆固醇饮食喂养8周后麦角新碱诱发血管痉挛情况。原位杂交和Northern　bloting法定位和定量检测各组血管eNOS　mRNA表达情况。结果　血管造影显示,内皮剥脱+胆固醇饮食组可诱导出局限性血管痉挛。原位杂交显示eNOS　mRNA定位在血管内皮细胞胞浆,痉挛血管节段内皮细胞内表达减少,Northern bloting法定量显示痉挛血管节段eNOS　mRNA相对表达量较其他组明显减少。结论　高胆固醇和内皮剥脱使eNOS基因转录和翻译下调,血管内皮eNOS　mRNA表达降低可能与实验性血管痉挛的诱发高度相关。Objective To investigate the relationship between endothelial NO synthase (eNOS) gene expression and experimental vasospasm. Methods Sixteen rabbits were randomly divided into 2 groups, which were subjected to balloon endothelial denudation with normal diet (n=8) or with hypercholesterol diet group (n=8). Angiography was performed to detect the vasospasm induced by ergonovine before and after denudation and 8 weeks after hypercholesterol feeding. In situ hybridization and Northern blotting were performed to localize and quantitate respectively the expression of eNOS mRNA. Results Visible vasospasm was induced at the denuded sites in rabbits with hypercholesterol diet, in which the expression of eNOS mRNA was detected in the endothelium by in situ hybridization at a lower level than that of rabbits with normal diet, as demonstrated by Northern blotting. Conclusion The decrement of eNOS mRNA expression resulted from balloon endothelial denudation and hypercholesterolemia may play an important role in the pathogenesis of experimental vasospasm.

关 键 词：冠状血管痉挛 基因表达 动物模型 内皮一氧化氮合酶 原位杂交 

分 类 号：R543.3[医药卫生—心血管疾病]