青心酮对肺动脉内皮细胞及红细胞生物力学特性的影响  

Effect of DHAP on biomechanic properties of pulmonary artery endothelial cells and erythrocytes

作　　者：黄云峰[1] 叶笃筠[2] 吴萍[2] 吴泽志[3] 

机构地区：[1]武警湖北总队医院麻醉科,湖北武汉430061 [2]华中科技大学同济医学院病理生理学教研室,湖北武汉430073 [3]重庆大学生物工程研究院,重庆400000

出　　处：《中草药》2002年第7期624-626,共3页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目 (No.3 0 0 70 92 9)

摘　　要：目的　观察青心酮对红细胞及肺血管内皮细胞生物力学特性的影响。方法　体外培养肺动脉内皮细胞 ,分对照组及青心酮大、中、小剂量处理内皮细胞 ,用微管吸吮技术观察细胞的变形过程。结果　与未用药组比较 ,在加用青心酮 30 min后 ,中、大剂量组内皮细胞弹性系数 K1 和 K2 增大 ,粘性系数μ变化不大 ;红细胞表观变形指数和最大变形值也明显增加。结论　青心酮可以增加肺血管内皮弹性 ,并使红细胞变形能力明显增强 。Object To observe the effects of 3,4 dihydroxyacetophenol (DHAP) on biomechanic properties of cultured pulmonary artery endothelial cells and isolated erythrocytes. Methods Cultured in vitro pulmonary artery endothelial cells were treated with large , middle and small doses of DHAP, and a control group was set up. The deformation process was observed using micropipette aspiration technique. Results Compared with the control group, the elastic coefficiency K 1 and K 2 of endothelial cells in large and middle dose groups increased significantly, while the viscosity coefficiency changed insignificantly in 30 min of giving DHAP. The apparent deformation index and maximum deformation value of erythrocytes also increased markedly. Conclusion DHAP can increase pulmonary blood vessel endothelial elasticity and erythrocyte deformability obviously, thus improving the blood circulation.

关 键 词：微管吸吮 实验研究 青心酮 肺动脉 内皮细胞 红细胞 生物力学 

分 类 号：R285.5[医药卫生—中药学]