跨界融合子Fhhh降解PTA废水mnp基因转录研究  

Transcription of mnp Gene in Inter-Kingdom Fusant Fhhh for Degradation of PTA Wastewater

作　　者：陈逊[1] 陈文权[1] 陶菁[1] 顾继东[2] 裴大平[1] 严俊[1] 郝春博[1] 程树培[1] 

机构地区：[1]南京大学污染控制与资源化研究国家重点实验室环境学院,南京210093 [2]香港大学生态学及分类学系

出　　处：《南京大学学报（自然科学版）》2002年第4期544-549,共6页Journal of Nanjing University（Natural Science）

基　　金：国家 8 6 3项目 (2 0 0 1AA2 14 191) ;江苏省自然科学基金 (BK990 33) ;香港中国环保工业有限公司资助

摘　　要：报道Fhhh的遗传稳定性 ,及其降解精对苯二甲酸 (PTA)废水过程中基因转录的研究结果 .聚合酶链式反应PCR的测定结果是 ,Fhhh同时含有mnp(来自黄孢原毛平革真菌 )、FL0 1(来自酿酒酵母真菌 )和 16SrDNA(来自土著细菌 )的 3个亲株的基因DNA片断 .表明Fhhh具有分子遗传稳定性 .反转录一扩增 (RT_PCR)的测定表明 ,Fhhh和黄孢原毛平革菌在降解PTA废水过程中 ,在Mn2 +、Cu2 +、Zn2 +、Se4 +4种金属元素优化水平的条件下 ,mnp基因均可转录 .研究结果为高效处理PTA废水 。The molecular genetic stability of the inter_kingdom fusant Fhhh and the transcription of mnp gene in degradation of the purified terephthalic acid (PTA) wastewater were studied in this paper. Fhhh contained 3 DNA fragments including mnp gene, FLOI gene and 16SrDNA demonstrated with polymerase chain reaction. The 3 DNA fragments were from the three parental strains, which were the fungous Phanerochaete chrysosporium , the fungous Saccharomyces cerevisiae , and the native bacterium strain YZI respectively. The transcription of mnp gene existing in both Fhhh and P chrysosporium could happen meaured with RT_PCR in degradation of PTA wastewater. Meanwhile 4 metal ions of Mn 2+ , Cu 2+ , Zn 2+ and Se 4+ in PTA wastewater were at optimal levels. The data suggested that the fact of mnp transcription of Fhhh in PTA wastewater should be favorable for setting up regulating system to increase treatment efficiency in molecular genetics.

关 键 词：跨界融合子 FHHH PTA废水 基因转录 mmp基因 FLO1 16SrDNA 精对苯二甲酸废水 比降解率 废水处理 生物降解 

分 类 号：X703[环境科学与工程—环境工程] X172