不同区域日本稻蝗Cytb基因序列及相互关系  

Mitochondrial DNA Sequences and Interrelationships ofOxya japonica from Different Areas in China

作　　者：任竹梅[1] 马恩波[1] 郭亚平[1] 

机构地区：[1]山西大学生命科学与技术学院,山西太原300006

出　　处：《山西大学学报（自然科学版）》2002年第3期244-248,共5页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金 (30 0 70 112 );山西省自然科学基金 (9910 96 )

摘　　要：对中国 7个不同地理区域的日本稻蝗共 19个个体的线粒体 DNA细胞色素 b基因中间部分序列进行测定 ,比较其同源性 ,计算碱基组成 ,并用中华稻蝗和景泰突颜蝗为外群构建不同单倍型的 NJ分子系统树。在获得的432 bp的序列中 ,A+T约占 70 .5 % ,其中 13个核苷酸位点存在变异 (约占 3.0 % ) ,4个位点引起了氨基酸的变异。就每个氨基酸密码子来看 ,第三位点的 A+T含量较高 ,达 87.4%。由系统树显示 ,6个单倍型共形成 3个聚类簇 。The mtDNA Cyt b fragments(432bp) in 19 individuals of Oxya japonica from 7 regions in China were sequenced.The sequences were compared each other,the base compositions were calculated and the molecular phylogenetic tree constructed by Neighbor-Joining methods.In the sequences,there appeared 6 haplotypes,in which A +T (%) average was made account for about 70.5 % in the total and there were 13 polymorphic sites(about 3.0 %),among which only 4 sites caused amino acid variation.The phylogenetic tree suggests that the different haplotypes from the 7 regions in China were grouped in 3 clusters and the branches of clusters had no directive relationships with the regional distribution.

关 键 词：日本稻蝗 MTDNA CYTB基因 地理分布 相互关系 线粒体DNA 细胞色素 虫害防治 序列测定 

分 类 号：S435.112.4[农业科学—农业昆虫与害虫防治] Q963[农业科学—植物保护]