桑青枯雷尔氏菌gsp G和gsp K基因的获取与生物信息学分析  

Acquisition and Bioinformatics Analysis of gsp G and gsp K Genes of Ralstonia solanacearum from Mulberry

作　　者：禚苏[1] 孔卫青[1] 

机构地区：[1]安康学院农学与生命科学学院/陕西省蚕桑重点实验室,陕西安康725099

出　　处：《山东农业科学》2015年第3期9-12,共4页Shandong Agricultural Sciences

基　　金：安康学院重点学科研究项目(zdxkzx007)

摘　　要：gsp G和gsp K参与青枯雷尔氏菌Ⅱ型分泌系统周质复合体的形成。本研究以桑青枯雷尔氏菌MR111为材料,利用PCR扩增和DNA测序技术成功获得其gsp G和gsp K基因438 bp和843 bp的序列,在线BLAST分析两个基因编码蛋白与其它青枯雷尔氏菌同源区域的相似性在90%以上。在线CDD分析两个蛋白除分别含有典型的T2SG和T2SK以及N端主要由亮氨酸和丙氨酸组成的分泌型信号肽外,gsp G还含Ⅳ型分泌系统的"Ⅳ_pilin_GFxxx E"结构,而在gsp K中则出现了重复的"GIQSTE"序列,该序列在雷尔氏菌属中高度保守。gsp G and gsp K genes are involved in the formation of periplasmic complex of Ralstonia so-lanacearum type Ⅱ secretion system .The sequences of gsp G and gsp K genes of Ralstonia solanacearum strain MR111 with 438 bp and 843 bp in length respectively were obtained from mulberry by PCR amplication and DNA sequencing technology .By online BLAST analysis , the similarity of the 2 genes with the homologous genes from other R.solanacearum species were both over 90%.There were typical functional structure of T2SG and T2SK, and secretory signal peptide composed mainly by leucine and alanine in the N -end in the 2 genes, respectively.A typeⅣsecretion system “Ⅳ_pilin_GFxxxE” structure was found in gsp G, and a re-peat sequence “GIQSTE” was found in gsp K.This repeat sequence was highly conserved in R.so-lanacearum.

关 键 词：桑树 青枯雷尔氏菌 Ⅱ型分泌系统 gsp基因 

分 类 号：S432.42[农业科学—植物病理学]