补肾中药对肾阳虚大鼠下丘脑-垂体-肾上腺轴、血淋巴细胞Ca^(2+)和血清钙的影响  

Effects of Tonifying Kidney Herb on Ca^(2+) of Hypothalamus-Pituitary-Adrenal Axis and Lymphocyte of Blood and Serum Calcium in Kidney-Yang-Deficiency Rats

作　　者：宋春风[1] 郑师陵[2] 吕佩源[2] 尹桂山[3] 李恩[3] 

机构地区：[1]河北医科大学电镜实验中心,河北石家庄050017 [2]河北省人民医院临床检验中心神经内科,河北石家庄050071 [3]河北医科大学生化教研室,河北石家庄050017

出　　处：《中国中医基础医学杂志》2002年第5期34-36,共3页JOURNAL OF BASIC CHINESE MEDICINE

摘　　要：目的 :研究细胞内Ca2 + 信号在肾阳虚发病中的作用及补肾中药的调整作用。方法 :用流式细胞仪及钙荧光探针Flou 3 AM对肾阳虚大鼠下丘脑 垂体 肾上腺轴、血淋巴细胞胞内Ca2 +([Ca2 + ]i)和血清钙进行测定。结果 :肾阳虚大鼠下丘脑和血淋巴细胞 [Ca2 + ]i及血清钙显著升高 ,补肾中药能够显著抑制下丘脑、血淋巴细胞内 [Ca2 + ]i和血清钙的升高。结论 :肾阳虚时下丘脑、血淋巴细胞 [Ca2 + ]i和血清钙的显著升高 ,导致Ca2 + 信号的异常 ,使下丘脑 垂体 肾上腺轴功能紊乱 ,免疫功能低下 ,出现肾阳虚的症状 ,补肾中药可能通过调整下丘脑、血淋巴细胞 [Ca2 +Objective:Study the effects of cytoplasmic Ca 2+ signal in kidney yang deficiency rat and regulation of tonify kidney herb.Methods:[Ca 2+ ]i of hypothalamus pituitary adrenal axis and lymphocyte of blood in rats were tested with flow cytometry by using [Ca 2+ ]i fluorescent probe Flou 3/AM,as well as serum calcium.Results:[Ca 2+ ]i of hypothalamus and lymphocyte of blood and serum calcium increased significantly in kidney yang deficiency rats.Tonifying kidney herb decreased [Ca 2+ ]i of hypothalamus and lymphocyte of blood and serum calcium.Conclusion:[Ca 2+ ]i of hypothalamus and lymphocyte of blood and serum calcium increased significantly during kidney yang deficiency,which induced abnormality of Ca 2+ signal.The change of Ca 2+ signal resulted the distrubance of function in hypothalamus pituitary adrenal axis and declination of immunity,and then the occurrence of symptom in kidney yang deficiency.Tonifying kidney herb probably exerted effect by regulating Ca 2+ of hypothalamus and lymphocyte of blood and serum calcium.

关 键 词：肾阳虚 淋巴细胞 CA^2+ 补肾中药 动物实验 

分 类 号：R285.5[医药卫生—中药学]