卡维地洛对大鼠缺血再灌注心肌超微结构的影响  

Effects of Carvedilol on Myocardial Ultrastructure in a Rat Model of Acute Myocardial Ischemia-reperfusion Injury

作　　者：曾玉杰[1] 傅丽英[2] 彭享胜[1] 冯义柏[1] 于世龙[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院心血管内科,武汉430022 [2]华中科技大学同济医学院药理教研室,武汉430030

出　　处：《医药导报》2002年第8期479-481,共3页Herald of Medicine

基　　金：湖北省"九五"重点攻关基金资助项目 (基金编号 :692P110 1)

摘　　要：目的 :评价第三代 β受体阻断药———卡维地洛 (CVD)对大鼠在体心脏缺血再灌注心肌的超微结构的影响。方法 :实验大鼠 2 4只 ,假手术组 8只 ,只开胸 ,不结扎左前降支冠状动脉 ,另外 16只通过结扎左前降支冠状动脉 ,建立大鼠在体心脏缺血再灌注损伤模型。后者又分为对照组 8只 ,未给药 ;CVD组 8只 ,术前 7d连续胃管给CVD(1mg·kg 1·d 1) ,结扎左前降支冠状动脉 3 0min后松开进行再灌注 6h ,处死动物。观察乳酸脱氢酶 (LDH)、肌酸磷酸酶(CK MB)、过氧化物酶 (MDA)变化并对心肌进行HE染色及电镜检查。结果 :CVD减少CK MB、LDH及MDA的释放。对照组心肌细胞HE染色及电镜检查呈明显片状或局灶性坏死 ,润盘结构紊乱 ,大量反应性的中性粒细胞浸润于坏死细胞之间 ;CVD组心肌细胞损伤程度明显减轻 ,粒细胞浸润也明显减少 ,润盘结构基本正常。结论 :CVD对大鼠在体缺血再灌注心脏具有明显保护作用。CVD有抗氧化、减少心肌梗死区中性粒细胞浸润。Objective:To appraise the in vivo effects of the 3rd generation βadrenergic receptor blocker carvedilol(CVD) on the myocardial ultrastructure in a rat model of acute myocardial ischemia reperfusion injury. Methods:8 rats of the treatment group were given each a daily dose of 1 mg·kg 1 of CVD through a stomach tube for 7 consecutive days before the induction of myocardial infarction. No drug was given in the 8 rats of the control group. Myocardial infarction (MI) was induced by ligation of the left anterior descending branch of the coronary artery(LAD) for 30 min. Untying of the ligation was followed by 360 min of reperfusion. The animals were sacrificed at the termination of reperfusion for further study. Serum lactate dehydrogenase (LDH), CK MB, and MDA were determined. Tissue samples removed from the infarcted areas were examined by light microscopy and electron microscopy. Results:In CVD treated rats, the release of LDH, CK MB, and MDA were reduced( P <0.05). In the myocardial tissue of the control animals, patchy or focal necrosis of myocytes, disorganization of intercalated discs and extensive infiltration of reactive neutrophil granulocytes among necrotic myocytes were demonstrated under the light microscope and electron microscope. In animals of the CVD treated group, the injury of myocytes as well as the infilhation of granulocylus was much less prominent and the structure of intercalated discs was basically normal. Conclusion:CVD was shown to provide an evident protective effect on ischemia reperfusion myocardium.

关 键 词：卡维地洛 大鼠 再灌注损伤 心肌保护 心肌缺血 超微结构 

分 类 号：R542.2[医药卫生—心血管疾病] R971[医药卫生—内科学]