HPLC测定左金丸中吴茱萸次碱含量  

Detemination of rutaecarpine In Zuojin Wan by HPLC

作　　者：潘敏翔[1] 刘昌叶[1] 常华[1] 赵淼[1] 姜韧[1] 

机构地区：[1]解放军306医院药剂科,北京100101

出　　处：《药学实践杂志》2002年第4期236-237,共2页Journal of Pharmaceutical Practice

摘　　要：目的:建立高效液相色谱法测定左金丸中吴茱萸次碱含量的方法。方法:采用高效液相色谱法。IntersilC_(18)分析色谱柱(4.6mm ID×250mm,粒径5μm),流动相为乙腈—10％乙腈(50:50),流速1ml·min^(-1),检测波长345nm。结果:吴茱萸次碱的理论板数为2100。回归方程Y=-0.2197+0.0000003484X(r=0.9999),线性范围12～60μm·ml^(-1)。平均回收率为96.9％(n=5),RSD 3.7％,最低检出浓度为0.lμg·ml^(-1)。结论:方法简便,结果准确。OBJECTIVE: A quantitative method was developed for the determination of rutaecarpine in Zuojin Wan by HPLC. METHOD:The chromatographic conditions include column Intersil C18, mobile phase: acetonitrile-10% acetonitrile ( 50: 50), flow rate 1 ml· min-1 , wavelength 345nm. RESULTS; The number of theoretical plates caculated by rutaecarpine peak was 2100, The regression equation of rutaecarpine was Y = -0.2197 + 0. 000000349X(r =0. 9999) and the liner range was 12 -60μg · ml-1 . The average recovery of rutaecarpine was 96. 9% (n =5) ,RSD 3. 7%. The measurable lowest limit of rutaecarpine was 0. 1μg ·ml-. CONCLUSION:The method is sensitive, rapid and accurate.

关 键 词：左金丸 吴芙萸次碱 高效液相色谱法 含量测定 

分 类 号：R286.0[医药卫生—中药学]