人羊膜细胞外基质与成纤维细胞体外培养的实验研究  

The experimental study of culture in vitro of fibroblasts seeded onto human amnion extracellular matrix(HA-ECM)

作　　者：何清义[1] 陈秉礼[2] 王智彪[2] 李起鸿[1] 

机构地区：[1]第三军医大学附属西南医院骨科,重庆400038 [2]重庆医科大学超声医学工程研究所骨科组

出　　处：《中华整形外科杂志》2002年第4期229-231,I004,共4页Chinese Journal of Plastic Surgery

摘　　要：目的　通过人羊膜细胞外基质 (HA ECM)与成纤维细胞体外培养 ,观察成纤维细胞在HA ECM上的生长特性 ,以及胶原原纤维产生情况 ,为使HA ECM成为皮肤组织工程化的载体打下基础。方法　将不同浓度的成纤维细胞种植于HA ECM上进行体外培养 ,通过倒置显微镜、光镜及透射电镜 ,观察细胞在HA ECM的生长、排列 ,以及细胞的粘附和胶原原纤维产生情况。结果　梭形的成纤维细胞在HA ECM上生长良好 ,呈放射状或平行排列 ;组织切片发现HA ECM上有较多梭形细胞附着 ,细胞排列紧密 ;电镜下见成纤维细胞胞膜基底部向HA ECM内伸出数个突起 ,细胞与羊膜基质粘附相当牢固 ,胞外有较多胶原原纤维堆积。结论　种植于HA ECM的成纤维细胞有一个最适宜的细胞浓度 (3 5× 10 6 个 /ml) ;HA ECM具有良好的支架和渗透作用 ,成纤维细胞在其上生长、粘附良好 ,胶原合成、分泌旺盛 ,HAObjective\ The aim of this experiment is to find proper cell carrier for skin tissue engineering. Methods\ Various concentration of fibroblasts were seeded onto HA ECM and cultured in vitro. The performance of cells' growth, array, adhesion and collagen secrection on HA ECM was observed with light microscope and transmission electron microscope. Results\ The fusiform fibroblasts oriented radiantly or longitudinally and closely packed onto the HA ECM, they attached firmly and proliferated to confluence on the stromal surface of HA ECM. Conclusion\ The optimal cell concentration is 3 5×10 6/ml, HA ECM is ideal carrier for fibroblasts because of its excellent scaffold and diffusion character istics. Futhermore it has the bioactive molecules such as fibronectin and laminin which play an important role in fibroblasts attachment and proliferation on HA ECM\;

关 键 词：人羊膜细胞外基质 成纤维细胞 体外培养 实验研究 

分 类 号：R318.0[医药卫生—生物医学工程]