带血供肌瓣作为骨形态发生蛋白载体修复骨缺损实验研究  

Experimental Study on Vascular Muscle Flap Combined With Bone Morphogenetic Protein for Forming Bone Bridge to Repair Bone Defect

作　　者：裴国献[1] 杨润功[1] 魏宽海[1] 金丹[1] 

机构地区：[1]第一军医大学南方医院创伤骨科,广州市510515

出　　处：《中国创伤骨科杂志》2001年第3期201-204,共4页

摘　　要：目的 探讨带血供肌瓣作为骨形态发生蛋白（BMP）载体修复骨缺损的可行性。方法 观察带血供肌瓣复合BMP和单纯BMP组修复骨缺损时的成骨情况,对四种不同载体的成骨能力进行比较。结果 以指深屈肌支为蒂制备的带血供肌瓣复合BMP修复骨缺损,效果优于单纯BMP组。带血供肌瓣联合纤维蛋白粘合剂复合BMP组修复骨缺损,效果优于其它载体。结论 带血供肌瓣可作为BMP的良好载体,带血供肌瓣联合纤维蛋白粘合剂作BMP的载体效果更优。Objective To investigate the effect of vascular muscle flap as a carrier of bone morphogenetic protein (BMP) to repair long bone defect. Methods Vascular muscle flap with BMP and BMP alone were implanted into the experimental models. Their conditions of new bone formation were observed and compared. Additionally bone defects were divided into 4 groups in random and repaired respectively with the vascular muscle flap combined with FS/BMP (group A) ?vascular muscle flap/BMP (group B) > bloodless muscle flap/BMP (group C) and autolyzed antigen-extracted allogeneic bone (AAA)/BMP (group D). Their a-bilities of bone forming were observed. Results In the group of vascular muscle flap combined with BMP, a large amount of cartilage was formed in the gaps of muscles by 3 weeks. The cartilage was absorbed and replaced by normal bone containing hematopoietic bone marrow by 6 weeks with obvious muscle cell atrophy. The wet bone weight of the new bone was 253. 52±20. 63 mg, which is significantly larger than that of the control group 172. 22±13. 95mg (P<0. 01). In group A, the cartilage formed by 3 weeks and woven bone formed by 6 weeks; the haversion system formed and muscle cells disappeared by 9 weeks. Narural bone was found and the Tmax measured with torsion test was 0. 82±0. 04n. m. The calcium content was 174. 55±5. 11 μg/g by 12 weeks. The ability of new bone formation in the 4 groups were ranged in the following order: group A was similar to group D, group A>group B>group C. Conclusion Vascular muscle flap can serve as an effective carrier for BMP. Vascular muscle flap combined with FS as carrier is better than vascular muscle flap as a carrier alone.

关 键 词：带血供肌瓣 骨形态发生蛋白 载体 骨缺损 修复 

分 类 号：R687.3[医药卫生—骨科学]