金鱼草胚珠中ATP酶活性的超微细胞化学定位  

THE ULTRACYTOCHEMICAL LOCALIZATION OF ATPASE ACTIVITY IN THE OVULES OF ANTIRRHINUM MAJUS L.

作　　者：何才平 杨弘远[1] 

机构地区：[1]武汉大学生物系,武汉430072

出　　处：《Acta Botanica Sinica》1991年第2期85-90,共6页Acta Botanica Sinica（植物学报：英文版）

基　　金：高等学校博士点专项资助项目基金

摘　　要：本文用磷酸铅沉淀技术对金鱼草(Antirrhinum majus L.)开花前2天和开花当天胚珠中 ATP 酶的超微细胞化学进行了研究。卵器没有酶活反应;中央细胞的质膜、细胞质和核质中均有酶活反应。胚囊壁与其外方角质层之间有一间隙层,其中含有一些絮状和小囊泡状物质,该层呈现强烈的酶活反应。合点端承珠盘组织的细胞核、质膜及其特殊的厚壁内有大量的酶,并通过合点端角质层的不连续区段与上述间隙层中的酶连成一片。一般珠被薄壁细胞仅质膜和胞间连丝上有酶活反应,但珠被绒毡层细胞核质中相当显著。根据 ATP 酶在胚珠中的分布特点,着重讨论了珠被绒毡层和承珠盘的功能,并推测胚囊壁外间隙层可能构成一个为胚囊提供养料的质外体运输系统。The ultracytochemical localization of ATPase activity was carried out by the method of lead precipitation in the ovules of Antirrhinum majus L.No ATPase activitv is observed in the egg apparatus,but some in the polar nuclei,cytoplasm and plasma membrane of the cen- tral cell.Between the embryo sac wall and the cuticle surrounding it,there is a gap where some filamentand vesicle-like structures were demonstrated by conventional staining method, and much of ATPase activity is found there.At the chalaza of the ovule,a lot of ATPase particles are found in the nuclei,plasma membranes and the thick and loose wall of the hy- postase cells.The particles of ATPase in the hypostase and those in the gap surrounding em- bryo sac are continuously distributed through the intervals of the cuticle at the chalazal end of the embryo sac.Some of ATPase particles are found on the plasma membranes and plas- madesmata of integument cells,noticeably much more in the nucleoplasm of the integumen- tary tapetum.According to the ATPase distribution pattern in the ovules,we suggest that the function of the integumentary tapetum and hypostase is secretion,and that the gap surround- ing the embryo sac may be an apoplastic channal for nutrient flow into the embryo sac.

关 键 词：金鱼草 胚珠 腺苷三磷酸酶 

分 类 号：Q949.781.1[生物学—植物学]