枸杞胚乳愈伤组织细胞的悬浮培养及无丝分裂的活体观察  

SUSPENSION CULTURE OF ENDOSPERM CALLUS CELL AND AMITOSIS IN VITRO

作　　者：顾淑荣[1] 桂耀林[1] 刘淑琼[1] 徐廷玉[1] 

机构地区：[1]中国科学院植物研究所,北京100044

出　　处：《Acta Botanica Sinica》1991年第6期478-481,共4页Acta Botanica Sinica（植物学报：英文版）

基　　金：中国科学院科学基金资助课题

摘　　要：The endosperm calli were induced on MS basic medium supplemented with lppm 2,4-D,0.5ppm KT and 5% sucrose.The medium which contained lppm BAP,0.1ppm NAA and 2%sucrose was used for cell suspension culture.In suspension cell culture,amitosis of cleavagedivision of nucleus have been observed after 5 days of culture.First the nuclear membraneand nucleolus disappeared.The crevice appeared in the center of the nucleus,and the nucleusdivided into two daughter nuclei of similar size and each with a nucleolus.The daughter nu-cleus resembled an eye in shape.FoIlowing;the emergence of cell wall,the two new unequalcells were produced.Such amitotic division proceeded repeatedly until the callus developedand eventually plantlet regenerated,The endosperm calli were induced on MS basic medium supplemented with lppm 2,4-D, 0.5ppm KT and 5% sucrose.The medium which contained lppm BAP,0.1ppm NAA and 2% sucrose was used for cell suspension culture.In suspension cell culture,amitosis of cleavage division of nucleus have been observed after 5 days of culture.First the nuclear membrane and nucleolus disappeared.The crevice appeared in the center of the nucleus,and the nucleus divided into two daughter nuclei of similar size and each with a nucleolus.The daughter nu- cleus resembled an eye in shape.FoIlowing;the emergence of cell wall,the two new unequal cells were produced.Such amitotic division proceeded repeatedly until the callus developed and eventually plantlet regenerated,

关 键 词：枸杞 胚乳 愈伤组织 细胞悬浮培养 

分 类 号：Q949.777.7[生物学—植物学]