低温吸胀对种子下胚轴细胞超微结构的影响  

EFFECT OF LOW TEMPERATURE DURING IMBIBITION ON ULTRASTRUCTURE IN HYPOCOTYLS

作　　者：陶宗娅[1] 邹琦[2] 程炳嵩[2] 

机构地区：[1]四川绵阳农业专科学校,绵阳 621000 [2]山东农业大学,泰安 271018

出　　处：《Acta Botanica Sinica》1991年第7期511-515,共5页Acta Botanica Sinica（植物学报：英文版）

摘　　要：大豆(Glycine max L.)与豌豆(Pisum sativum L.)种子分别在25℃或5℃的水中吸胀4.5小时,下胚轴细胞质膜和线粒体外膜已基本恢复连续。两类种子在5℃吸胀3小时后置25℃下萌发6天,下胚轴细胞质膜出现断裂等异常结构,未分解的脂肪体和蛋白体仍大量存在;大豆种子线粒体内嵴很少,粗糙内质网等内膜系统发育很不完善,豌豆种子线粒体已形成了较多内嵴。吸胀冷害的一个重要原因很可能是低温吸胀阻碍了生物膜结构与功能重建,尤其是线粒体结构与功能的完整化进程。Soybean (chilling-sensitive) and pea (chilling-insensitive) seeds imbibed at 25℃ or 5℃ for 4.5h, and hypocotyls were removed, fixed nd examined for detecting the effect of temperatures during imbibition on membrane structures, It was shown that plasma membranes and external membranes in mitochondria in both seeds had even repaired their continuity. When the seeds of the two species germinated at 25℃ for 6 days after imbibed at 5℃ for 3h, parts of plasma membranes in hypocotyl cells were brokendown and sunken, there were a number of undegraded lipid bodies and protein bodies, in particular, the inner crista in mitochodria in soybean seed nearly failed to develop, and there were few Golgi bodies and rough ER in contrast to the controls and peas. It is suggested that it may be an important cause of imbitional chilling injury that reorganization of biomembrane structures and functions and the functional and structural integrity of mitochodria in particular were imbibited due to low temperature during imbibition.

关 键 词：大豆 豌豆 种子 吸胀温度 超微结构 

分 类 号：S311[农业科学—作物栽培与耕作技术]