菠菜甜菜碱醛脱氢酶对甜菜碱醛的氧化  

OXIDATION OF BETAINE ALDEHYDE BY BETAINE DEHYD-ROGENASE FROM SPINACH LEAVES

作　　者：梁峥[1] 赵原[1] 李浴春 邹喻苹[1] 骆爱玲[1] 黄巨富[1] 

机构地区：[1]中国科学院植物研究所

出　　处：《Acta Botanica Sinica》1991年第9期680-686,共7页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金;国家科技攻关计划资助课题

摘　　要：测定了15种植物叶片的甜菜碱含量。结果表明,抗盐碱、干旱和抗寒的植物含量较高。从菠菜叶片分离和部分纯化了甜菜碱醛脱氢酶,并对其某些性质进行了研究。甜菜碱醛脱氢酶存在于60%的硫酸铵部分,70%硫酸铵部分未检出其活性。该酶有两个同工酶,在-80℃条件下十分稳定,其活性为0.125—1.0mol/L的NaCl和KCl所抑制,但Mn^(2+)和Mo^(6+)离子对活性没有影响,而Mg^(2+)稍增加其活性。Betaine content in leaves of fifteen plant species was determined.The results showed higher betaine levels in those salt-,drought-,and chilling-resistant species.Betaine aldehyde dehydrogenase (BADH,EC 1.2.1.8) was isolated and partially purified from spinach leaves. Some properties of this enzyme were studied.BADH was precipitated by 60% saturation of (NH_4)_2SO_4.Its activity was not detected in 70% saturation of (NH_4)_2SO_4.BADH has two isoenzymes.The activity of BADH was quite stable below-80℃.It was inhibited by 0.125- 1.0 mol/L NaCl or KCl,but not by Mn^(2+) and Mo^(6+),and slightly increased by Mg^(2+).

关 键 词：菠菜 甜菜碱醛 脱氢酶 氧化 

分 类 号：S636.101[农业科学—蔬菜学]