微载体培养Vero细胞工艺条件初探  

Study on Vero Cell Culture Technique with Microcarriers

作　　者：徐殿胜[1] 董树沛 华平[1] 王国政[1] 俞俊棠[1] 宋家骊[2] 陈列胜[2] 贺适民 

机构地区：[1]华东化工学院生化工程研究所 [2]卫生部上海生物制品研究所

出　　处：《华东化工学院学报》1989年第4期441-446,共6页

摘　　要：本文研究了细胞生长过程中微载体的种类,浓度及细胞的接种量、溶解氧水平对培养Vero细胞的影响。在pH为7.0～7.4,液解氧水平为30%～50%空气饱和浓度,搅拌速度为25～35r/min,温度为37℃时,在1.5升Celligen^(TM)系统中,连续培养7天,可使细胞浓度达到2.5×10~6 Cells/ml,为接种量的12倍,细胞生长旺盛,形态正常。Several factors which may affect the growth of Vero cell have been studied. The kind and concentration of microcarriers, cell inoculation density and dissolved oxygen level can influence cell growth substantially. Under the following conditions: pH=7.0～7.4; dissolved oxygen, 30%～50% saturated air; stirring speed, 25-35r/min; and temperature 37℃, cell concentration can reach 2.5×10~6 cells/ml after 7 days culture in the 1.5 L CELLIGEN system. The resulting cell concentration is almost 12 times as high as the initial cell inoculation density.

关 键 词：细胞培养 VERO细胞 微载体 溶解氧 

分 类 号：Q954.6[生物学—动物学]