鸡马立克氏病火鸡疱疹病毒(耐热保护剂)活疫苗的研究  

Study on Thermo-tolerant Freeze-dried Vaccine of Turkey Herpesvirus(HVT)

作　　者：王栋[1] 刘业兵[1] 张万林 刘效文[1] 张仲秋[1] 王利永[1] 

机构地区：[1]中国兽医药品监察所,北京100081 [2]辽宁益康生物药品厂,辽宁辽阳111000

出　　处：《中国兽药杂志》2002年第7期27-29,共3页Chinese Journal of Veterinary Drug

基　　金：攀登计划B类项目(课题编号85-44-03-09);科技部科技型中小企业技术创新基金资助项目

摘　　要：通过对不同保护剂基质与鸡马立克氏病火鸡疱疹病毒（HVT）相溶性的研究及冻干曲线的优化，成功研制出一种适宜HVT的耐热冻干保护剂，用其与相匹配的48h自然回温冻干曲线冻干的HVT耐热活疫苗，其病毒冻干损失比SPGA常规疫苗减少约50%，在2-8℃条件下可保存24个月，37℃保存10d病毒存活率达50.5%，而SPGA规程苗仅为31.6%，经约50万羽份的田间试验，证明安全有效并显示出良好的耐热性能，达到国外同类产品水平。A new stabilizer was studied with different suspending media and freeze-drying system for HVT vaccine. It wasshowed that the highest virus survival ratio in freeze-drying procedure to HVT. The vaccine could be stored for 24 monthsat 2~8℃. After stored at 37℃ for 10 days, the virus survival ratio was 50%, comparing with the control 31.6%. After about500,000 doses of vaccine used in poultry farms, the results showed that the vaccine was effective and safe to protectchickens against Marek’s Disease.

关 键 词：鸡马立克氏病 火鸡疱疹病毒 耐热保护剂 活疫苗 耐热冻干保护剂 

分 类 号：S858.315.3[农业科学—临床兽医学] S859.797[农业科学—兽医学]