马铃薯Y病毒两分离物特性及其侵染寄主的超微结构比较研究  

Chracterization on two isolates of potato virus Y and ultrastructural alteration of host cells infected with the two isolates.

作　　者：郭兴启[1] 李向东[2] 王秀芳[2] 竺晓平[2] 温孚江[2] 

机构地区：[1]浙江大学生物技术研究所,浙江杭州310029 [2]山东农业大学植物抗病毒基因工程实验室,山东泰安271018

出　　处：《浙江大学学报（农业与生命科学版）》2002年第4期411-416,共6页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金资助项目 (39970 4 85 )

摘　　要：报道了马铃薯 Y病毒两分离物的生物学和血清学特性、病毒粒体形态大小以及它们引起的寄主细胞病变特征等方面的差异。从山东省主要烟草和马铃薯种植区病毒样品中分离到两个马铃薯 Y病毒分离物 ,分别为 PVY- T和 PVY- P。两分离物在寄主植物上的症状表现、体外抗性、蚜传特性、血清学以及病毒粒体大小等方面存在着明显的差异。同时 ,这些结果与作对照的 PVY标准株系 PVYN和PVYO相比较 ,初步推测分离物 PVY- T属于 PVYO 株系 ,而 PVY- P则属于 PVYN。通过对受侵染寄主的细胞超微结构观察比较发现 ,这两种 PVY分离物在寄主细胞内的分布特征、内含体以及与寄主互作后所引起的细胞病变也明显不同 ,这些可能成为区别 PVY不同株系的依据之一。The present paper gives detailed description about the differences of two isolates of potato virus Y (PVY) in biological properties, morphology of purified virus particle, serological relationship and the ultrastructural alterations in infected tobacco. The two isolates ( i.e., PVY T and PVY P) were obtained from tobacco and potato fields in Shandong province, respectively. Based on tests on the host ranges and symptoms on different hosts, the stability in sap in vitro, aphid transmissibility and serological properties, the PVY T isolate is proved belonging to the PVY O strain group, while the PVY P isolate was confirmed similarity to PVY N. Meanwhile, the ultrastructural alterations caused by the infection of PVY T and PVY P were also investigated. Our results showed that the cytopathological effects of PVY T differed obviously from that of PVY P. These characteristic differences might be useful in the virus diagnosis and identification of PVY strains.

关 键 词：马铃薯Y病毒 分离物特性 侵染寄主 超微结构 比较研究 

分 类 号：S432.41[农业科学—植物病理学] S435.32[农业科学—农业昆虫与害虫防治]