费氏中华根瘤菌内源质粒和大豆品种对菌株竞争结瘤能力及固氮效率的影响  

The Influence of Indigenous Plasmids and Soybean Cultivar on the Competitiveness for Nodules of the Strains and Nitrogen-fixing Effectiveness of Sinorhizobium fredii

作　　者：缪礼鸿[1] 周俊初[1] 郑慧芬[1] 

机构地区：[1]农业部农业微生物重点实验室华中农业大学,武汉430070

出　　处：《中国农业科学》2002年第7期802-808,共7页Scientia Agricultura Sinica

基　　金：国家"8 63"计划 (2 0 0 1AA2 14 0 2 1)课题;欧盟INCO DC合作研究计划ICA4 2 0 0 0 10 13 7课题经费资助项目

摘　　要：采用发光酶基因 (luxAB)标记技术 ,在无菌砂培条件下测定了费氏中华根瘤菌 (Sinorhizobiumfredii)内源质粒和大豆品种对供试菌株竞争结瘤和固氮效率的影响。结果表明 ,供试菌株的固氮效率主要由共生质粒所决定 ;而竞争结瘤能力主要取决于其染色体所携带的基因 ,内源质粒 (包括共生质粒和非共生质粒 )对供试菌株竞争结瘤能力没有明显的影响。菌株的竞争结瘤能力对供试的大豆品种依赖性较小 。The Influence of indigenous plasmids and soybean cultivar on competitiveness for nodules of the strains and symbiotic effectiveness of Sinorhizobium fredii strains marked by luxAB genes were tested by pot experiments. The results indicated that nitrogen fixing efficiency of the tested strains mainly depended on symbiotic plasmids and the competitiveness of nodulation mainly on the chromosome genes of strain, the indigenous plasmids (including symbiotic and non symbiotic plasmid) and soybean cultivar shown no significant influence on competitiveness of nodulation of the strains tested.

关 键 词：费氏中华根瘤菌 内源质粒 大豆 品种 竞争结瘤能力 固氮效率 

分 类 号：S154.3[农业科学—土壤学]