人TLR4的B细胞优势表位设计、合成及其免疫原性检测  

Predication and synthesis of B cell epitopes of human toll-like receptor 4 (TLR4) and determination of its immunogenicity

作　　者：杨清武[1] 朱佩芳[1] 王正国[1] 吕凤林[1] 蒋建新[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所四室,重庆400042

出　　处：《中华微生物学和免疫学杂志》2002年第4期362-365,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家"973"资助项目 (G19990 5 42 0 3) ;全军医药卫生科研基金资助项目 ( 0 1Q10 5 )

摘　　要：目的　鉴定TLR4的优势抗原表位 ,为抗人TLR4单克隆抗体制备奠定基础。方法　分别应用Hoop&Woods亲水性参数、抗原性参数、可及性参数和抗原表位的软件分析对TLR4B细胞表位进行预测 ,最后用抗原性指数的方法进行综合评述。合成针对该表位的多肽 ,以此多肽为免疫原免疫小鼠 ,对其免疫原性进行检测。结果　预测TLR4的B细胞优势表位为 189～ 2 0 2氨基酸序列 :NH2 HKLTLRNNFDSLNV COOH ,此多肽能诱导机体产生较高的抗体滴度 ,多抗具有较高的特异性。结论　预测的TLR4短肽是B细胞的优势表位 ,以此制作抗人TLR4单克隆抗体是可行的。Objective To identify B cell dominant epitope of TLR4 and to provide research basis for developing monoclonal antibody for TLR4. Methods B cell dominant epitopes of TLR4 were predicated by software of Hoop & Woods hydrophility, antigenicity (Welling), accessibility (Janin) and epitope and were evaluated with anverage antigenicity index. The peptide of the epitopes was synthesized and BABL/c mice were immunized and thereby its immunogenicity was determined. Results The NH2-HKL TLR NNF DSL NV-COOH was the dominant epitope of the B cells. Synthesized peptides induced high titer of antibody and the polyclonal antibody to TLR4 has high specificity. Conclusion The peptides could be used for preparation of site-directed monoclonal antibody against TLR4.

关 键 词：B细胞优势表位 分子设计 TLR4单克隆抗体 抗原性指数 抗体制备 免疫原性 

分 类 号：R392[医药卫生—免疫学]