复合脂质体对大鼠肠缺血再灌注保护作用的研究  

Protective effects of liposomes containing L-arginine,Se and taurine on intestinal ischemia-reperfusion injury in rats

作　　者：周水生[1] 罗德生[1] 高卉[1] 万敬之[1] 雷平[1] 

机构地区：[1]湖北省咸宁医学院外科学教研室,湖北咸宁437100

出　　处：《中国病理生理杂志》2002年第7期844-846,I004,共4页Chinese Journal of Pathophysiology

摘　　要：目的 :探讨人工合成的富含L -精氨酸、亚硒酸钠、牛磺酸的复合脂质体对抗大鼠肠缺血再灌注损伤的作用及机制。方法 :Wistar大鼠随机分为假手术组、缺血再灌注组 (I/R)、缺血前给药组和再灌时给药组。夹闭大鼠肠系膜上动脉 (SMA) 6 0min造成缺血 ,松夹即为再灌注 ,再灌注 2h后分别测定各组肠组织丙二醛 (MDA)含量、T-SOD活性及Ca2 + -Mg2 + -ATP酶活性 ,并观察各组肠组织超微结构及bcl- 2蛋白的表达情况。结果 :缺血前给药组和再灌时给药组MDA含量均明显低于缺血再灌注组 (P <0 0 1) ,T -SOD和Ca2 + -Mg2 + -ATPase活性均明显高于缺血再灌注组 (P <0 .0 1) ,bcl- 2蛋白在正常组适当表达 ,在缺血再灌注组不表达 ,而缺血前给药组和再灌时给药组均表达强阳性 (P <0 0 1) ,且两用药组间各项指标比较无明显差异 (P >0 0 5 )。结论 :复合脂质体可能通过抑制脂质过氧化、稳定内环境、诱导bcl-AIM and METHODS: To study the protective effects of liposomes containing L-Arg,Se and taurine on intestinal ischemia-reperfusion injury in rats. Wistar rats were divided randomly into sham operated group,ischemia-reperfusion(I/R) group,pretreatment with liposomes group and treatment with liposomes at reperfusion group. In the experiments, superior mesenteric artery was clipped for 60 min, and then unclipped. 2 hours of reperfusion later, MDA content, T-SOD and Ca 2+ -Mg 2+ -ATPase activities in intestinal tissues were detected respectively, ultrastructure and bcl-2 expression in intestinal mucosa tissue were observed. RESULTS: MDA content in liposomes-treated group was less than I/R group ( P <0.01).The activities of T-SOD and Ca 2+ -Mg 2+ -ATPase in liposomes-treated group were higher than I/R group ( P <0.01). Bcl-2 staining was negative in I/R group, and was positive in liposomes-treated group (P <0.01).There was no difference in above indexes between pretreatment with liposomes group and treatment with liposomes at reperfusion group( P >0.05). CONCLUSION: Liposomes containing L-arginine, Se and taurine can protect intestine against ischemia-reperfusion injury in rats,which may be related to inhibiting lipid peroxidation, stabilizing internal circumstances and inducing bcl-2 protein expression.

关 键 词：复合脂质体 肠缺血 保护作用 肠缺血再灌注损伤 

分 类 号：R656[医药卫生—外科学]