银杏LEAFY同源基因的分离与克隆  

LEAFY HOMOLOGOUS GENE CLONED IN MAIDENHAIR TREE(GINKGO BILOBA L.)

作　　者：张建业[1] 陈力耕[1] 胡西琴[1] 何新华[2] 

机构地区：[1]浙江大学园艺系,杭州310029 [2]广西大学园艺系,南宁530005

出　　处：《林业科学》2002年第4期167-170,共4页Scientia Silvae Sinicae

基　　金：国家自然科学基金资助项目 (批准号 :3 0 0 70 63 4)

摘　　要：The flower meristem identity gene LEAFY is a developmental switch,sufficient for flower initiation in diverse plants. Ginkgo biloba is a kind of gymnosperm,and a dioecious tree species.The nucleotide DNA sequence was cloned from the genomic DNA of the tender leaves collected from a male tree in Ginkgo biloba L.cv.Dafushou.The analysis of DNA sequence structure indicated that the gene was composed of 3450 bp,and inferred the sequence to LEAFY gene of the male Ginkgo .Comparing the gene with LEAFY gene of the female Ginkgo reported in the relative references,the rate of homology was 99%,only lacking 3 nucleotides.The research work laid a sound foundation for studing the regulation and controlling flowering mechanism in Ginkgo biloba L.at the molecuar level in the future.The flower meristem identity gene LEAFY is a developmental switch,sufficient for flower initiation in diverse plants. Ginkgo biloba is a kind of gymnosperm,and a dioecious tree species.The nucleotide DNA sequence was cloned from the genomic DNA of the tender leaves collected from a male tree in Ginkgo biloba L.cv.Dafushou.The analysis of DNA sequence structure indicated that the gene was composed of 3450 bp,and inferred the sequence to LEAFY gene of the male Ginkgo .Comparing the gene with LEAFY gene of the female Ginkgo reported in the relative references,the rate of homology was 99%,only lacking 3 nucleotides.The research work laid a sound foundation for studing the regulation and controlling flowering mechanism in Ginkgo biloba L.at the molecuar level in the future.

关 键 词：LEAFY同源基因 分离 克隆 银杏 雄株 童期 开花 分子调控机理 

分 类 号：S664.3[农业科学—果树学]