精氨酸加压素C端片段(4～8)对大鼠海马神经元CCT的影响(英文)  

Argipressin(4—8) Upregulate CTP: Phosphocholine Cytidylyltransferase in Rat Hippocampal Neurons

作　　者：徐侃彦[1] 熊樱[1] 杜雨苍[1] 

机构地区：[1]中国科学院上海生命科学研究院生物化学与细胞生物学研究所,上海200031

出　　处：《生物化学与生物物理学报》2002年第4期418-422,共5页

基　　金：国家重点基础研究规划 (973 )项目资助 (No .G19990 5 40 0 0 )~~

摘　　要：为了研究精氨酸加压素C端片断 4～ 8(AVP4～ 8)对大鼠海马神经元中胞苷三磷酸 :磷酸胆碱胞苷转移酶 (CCT)的mRNA水平和酶活性的影响及其作用机制。将大鼠海马神经元用不同药物处理一定时间后 ,用RT PCR结合Southern杂交的方法测定CCT的mRNA水平 ,用测定胞苷二磷酸胆碱中 [14 C]的参入率来确定CCT活性。结果表明 :AVP4～ 8能上调海马神经元内CCT的mRNA水平 ,AVP4～ 8的拮抗剂ZDC(C)PR对此有抑制作用 ,当用放线菌素D中止基因转录 ,发现用AVP4～ 8共处理的细胞 ,比单独用放线菌素D处理的细胞 ,其CCTmRNA的半衰期较长 ;AVP4～ 8处理后 ,神经元内CCT的酶活性也有一定程度的上升。由此得出结论 :AVP4～ 8通过提高神经元内CCTmRNA的稳定性 ,提高了CCTmRNA的水平 ,进而影响了胞内CCT的酶活性。In order to study the effect of argipressin(4—8)(AVP 4—8 ) on the mRNA level and activity of cytidine triphosphate: phosphocholine cytidylyltransferase(CCT) in rat hippocampal neurons, and elucidate its possible mechanism. Rat hippocampal neurons treated with AVP 4—8 or actinomycin D were incubated with different time periods. The mRNA level of CCT was detected using RT PCR plus Southern blot, CCT activity was determined by measuring the rate of incorporation of [ 14 C] phosphocholine into cytidine diphosphate choline(CDP choline). It was found that AVP 4—8 could upregulate the CCT mRNA in rat hippocampal neurons. ZDC(C)PR, the antagonist of AVP 4—8 , could greatly inhibit this upregulation. Using actinomycin D to inhibite the eucaryotic transcription, it was found that the halflife of CCT mRNA could be prolonged by coincubation with AVP 4—8 . Meanwhile, AVP 4—8 could also increase CCT activity in rat hippocampal neurons. These results demonstrated that AVP 4—8 upregulated CCT mRNA level and its activity through stabilizing the CCT mRNA in rat hippocampal neurons.

关 键 词：精氨酸加压素C端片段 大鼠 海马神经元 CCT 磷酸胆碱胞苷转移酶 磷脂酰胆碱 放线菌素D 

分 类 号：Q555[生物学—生物化学]