羊草种群遗传分化的RAPD分析Ⅱ.RAPD数据的统计分析  

Genetic Differentiation in Leymus chinensis Populations Revealed by RAPD Markers Ⅱ. Statistics Analysis

作　　者：崔继哲[1] 祖元刚[1] 聂江城[1] 

机构地区：[1]东北林业大学森林植物生态学重点实验室

出　　处：《生态学报》2002年第7期982-989,共8页Acta Ecologica Sinica

基　　金：国家教委跨世纪优秀人才计划基金项目

摘　　要：对松嫩草原上分布的灰绿型和黄绿型羊草 9个种群进行了 1 5个引物的 RAPD分析。统计结果表明 ,两类种群的扩增片段数和多态位点比率明显不同 ,黄绿型种群低于灰绿型 ,其值分别 <90与 >1 0 0 ,<5 0 %与 >70 %。比较了 7种不同统计方法据 RAPD表型或基因型频率估算的种群遗传多样性 ,几种统计结果都揭示 ,黄绿型种群低于灰绿型种群。用 FIS值矫正种群对 Hardy-Weinberg平衡的偏离后 ,估算等位基因频率 ,通过 Shannon指数和 Nei指数估计羊草种群间分化分别为 37.6 %和 35 .7% ,高于等位酶的分析。讨论比较了等位酶和Leymus chinensis (Trin.)Tzvel., which is one of rhizome grass species, has two ecotypes in the Songnen Plain,including a gray green type and a yellow green type. The levels of genetic diversity and genetic differentiation based on RAPDs were assessed among seven populations of the gray green type and two populations of the yellow green type (a total of 105 individuals). Fifteen 10-mer primers screened from seventy Operon's primer series were used to amplify RAPD fragments using a standard RAPD protocol. The number of amplified fragments and the percentage of polymorphic loci were different among nine populations. Populations that belong to the yellow green type possessed fewer amplified fragments (<90) and lower percentage of polymorphic loci(<50%)than that of the gray green type (>100 and >70% respectively). Genetic diversity based on RAPD phenotypic or genotypic frequencies was calculated using seven published methods by the statistical package RAPDISTANCE. The genetic diversity level in the yellow green type was lower than that in the gray green type. F_ IS value previously estimated with codominant markers, e.g.allozymes for the same population was used to estimate the null-allele frequencies for putative RAPD loci corrected for potential deviations from Hardy-Weinberg equilibrium. Shannon's information index and Nei's index of genetic diffferentiation were used to partition diversity within and among population components, and 37.6% or 35.7% of the variation detected with RAPDs was partitioned among populations. Genetic identity between the populations of two types were both on 0.8, while the genetic indentity within the gray green populations was above 0.9. The results achieved by the methods of either allozymes previously adopted or RAPDs revealed the same genetic pattern of variation. These results at the DNA level were parallel to those at the protein level. At both the protein and DNA levels, genetic polymorphism was higher in the populations of the gray green type than that in the yellow green populat

关 键 词：羊草种群 遗传分化 RAPD分析 数据 统计分析 遗传多样性 草原 

分 类 号：S543.901[农业科学—作物学]