转反义PG基因番茄果实细胞结构变化的研究  

A Study on Changes in Cell Structure of Antisense PG Transgenic Tomato Fruit

作　　者：李曜东[1] 顾淑荣[1] 魏玉凝[1] 周馥[1] 赵敬芳[1] 

机构地区：[1]中国科学院植物研究所,北京100093

出　　处：《植物学通报》2002年第3期348-353,共6页Chinese Bulletin of Botany

基　　金：国家自然科学基金资助项目 (批准号 :3 9770 5 3 2 )

摘　　要：经细胞学观察发现 ,转反义PG基因番茄果实在不同成熟期及存放前后 ,其果皮外面几层细胞的厚度都比未转基因的厚 1～ 5 μm ,细胞结构、细胞质和细胞核等的状态都有明显区别。尤以贮存后更为明显 ,未转基因果实的果皮细胞结构解体、细胞质凝聚、细胞核变的模糊程度都比转基因的严重。经外源乙烯处理后 ,转基因和未转基因果实的细胞结构也有相似的变化。结果表明 :反义PG基因的转入降低了PG活性 ,并且减弱了外源乙烯的作用 ,延缓了果实的衰老 ,提高了耐贮性能 。It was observed that the outer_layer cells of the pericarp of antisense PG transgenic tomato were 1～5 μm thicker than those of nontransgenic tomato, and clear differences existed in cell structure and cytoplasm and nucleus status between the fruits of the transgenic and nontransgenic tomatoe at different stages of fruit ripening and storage, especially at the end of storage. The fruit of nontransgenic tomato showed a serious collapse of pericarp cell structure, condensation of cytoplasm and blurring of nucleus, compared with that of the transgenic tomato. Exogenous ethylene treatment caused similar changes in cell structure of the fruits of transgenic and nontransgenic tomatoes. These results demonstrated that the expression of the antisense PG gene in tomato fruit effectively inhibited the activity of endogenous PG, reduced the effect of ethylene and prolonged the time of fruit storage.

关 键 词：转反义PG基因番茄 果实细胞结构 转基因番茄 乙烯 

分 类 号：S641.2[农业科学—蔬菜学]