三倍体毛白杨组织脱分化培养与植株体再生  

INDUCTION,DIFFERENTIATION AND PLANT REGENERATION OF TRIPLOID CALLI OF POPULUS TOMENTOSA

作　　者：李毅[1] 何明珠[1] 马海芸 

机构地区：[1]甘肃农业大学林学院,兰州730007 [2]榆中县林业技术中心,兰州730010

出　　处：《植物研究》2002年第3期288-291,共4页Bulletin of Botanical Research

基　　金：国家科技攻关 (2 0 0 1BA90 1A3 9);甘肃省教委资助 (9780 5 70 )项目

摘　　要：采用毛白杨三倍体幼叶作为外植体进行组织培养 ,以MS为基本培养基获得了再生植株。从NAA和IAA与 6 BA间进行的 1 8个正交试验中 ,选择出适宜脱分化培养基MS + 6 BA0 .5mg L +NAA0 .1mg L ,对三倍体毛白杨愈伤组织诱导率为 87.6%。 5种生长素与 6 BA配比的再分化培养基中 ,1 2MS +IAA0 .1mg L + 6 BA0 .1mg L对不定芽的分化诱导率可达到 68.5 % ;MS +6 BA0 .5mg L +NAA0 .0 1mg L的培养基可使单芽直接增殖出 7.4个芽。在MS +IBA 1 .0mg L的生根培养基上 ,试管苗的生根率可达 85 .7%。Regenerating plants were produced from immature leaves of the triploid of Populus tomentosa by tissue culture on MS basic medium with different plant hormone. The result showed that callus induction frequency reached to 87.6 percent on MS+6 BA 0.5 mg/L + NAA0.1 mg/L ; differentiation frequency reached to 68.5 percent on 1/2 MS + IAA0.1 mg/L + 6 BA0.1. It can be generated from one to 7.4 axillary shoots on MS+6 BA0.5 mg/L +NAA0.01 mg/L . Rooting frequency of the shoots got to 85.7 percent on MS+ IBA 1.0mg/L

关 键 词：三倍体毛白杨 组织脱分化培养 植株体再生 愈伤组织 生根 

分 类 号：S792.117[农业科学—林木遗传育种] S723.132[农业科学—林学]