体外分子定向进化研究进展  

Recent Advances in In vitro Molecular Directed Evolution

作　　者：徐卉芳[1] 张先恩[1] 张用梅[1] A.E.G.CASS 

机构地区：[1]中国科学院武汉病毒研究所,武汉430071 [2]ImperialCollegeofScience,TechnologyandMedicine,SouthKensington,LondonSW72AY,UK

出　　处：《生物化学与生物物理进展》2002年第4期518-522,共5页Progress In Biochemistry and Biophysics

基　　金：中国科学院与英国皇家学会联合资助项目 (Q174)~~

摘　　要：体外定向进化作为近几年发展起来的一种蛋白质改造新策略 ,可以在未知目标蛋白三维结构信息和作用机制的情况下 ,通过对编码基因的随机突变、重组和定向筛选 ,获得具有改进功能或全新功能的蛋白质 ,使几百万年的自然进化过程在短期内得以实现 ,因而是发现新的生物活性分子和反应途径的重要方法 ,已在短短几年内取得了令人瞩目的成就 .In vitro molecular directed evolution, based on the combination of random mutagenesis, recombination and high throughput screening, is a new strategy of improving protein properties. With this technology, the properties of the target proteins, such as specificity, catalytic activity and affinity can be tuned without the knowledge of 3 D structure information and function mechanisms. Recently, the methodology originating from error prone PCR and DNA shuffling has been greatly developed by many new techniques. Here, based on the literature review and the experience of authors, the recent developments have been reviewed.

关 键 词：体外分子定向进化 研究进展 体外分子进化 蛋白质工程 渐进切割法产生杂和酶 基因组进化 

分 类 号：Q81[生物学—生物工程]