HBV包膜-核心蛋白融合基因DNA疫苗诱导小鼠持久的细胞免疫应答  

Strong and Durable Cellular Immune Responses in Mice Induced by Hepatitis B Virus Envelope and Core Fusion Gene DNA Vaccine

作　　者：赵平[1] 姜春鹏[2] 赵兰娟[1] 温新宇[1] 戚中田[1] 

机构地区：[1]第二军医大学微生物学教研室,上海200433 [2]东北农业大学生物工程系,哈尔滨150030

出　　处：《生物化学与生物物理进展》2002年第4期576-582,共7页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目 (3 9980 0 3 8);上海科技发展基金资助项目 (0 0 43 192 0 6) ~~

摘　　要：构建编码HBV包膜 核心蛋白融合基因的DNA疫苗pSC、pSS1S2C和编码HBV包膜蛋白或核心蛋白基因的DNA疫苗 pHBs、pHBc ,分别肌肉注射免疫BALB/c小鼠 ,检测小鼠的血清抗体、T细胞增殖和细胞毒性T淋巴细胞反应 ,比较融合基因DNA疫苗与单基因DNA疫苗诱生免疫应答的强度 ,发现融合基因DNA疫苗诱生抗体的效率明显不及单基因DNA疫苗 ,但其能诱导更强、更持久的细胞免疫应答 ,表明HBV包膜 核心蛋白融合基因DNA疫苗对于治疗慢性乙型肝炎可能比单基因DNA疫苗更为有效 .Four kinds of hepatitis B virus(HBV)DNA vaccines were constructed, two of which encode HBV envelope and core fusion gene,and the others encode HBV envelope or core gene. These DNA vaccines were intramuscularly vaccinated into BALB/c mice respectively. The serum antibodies, T lymphocyte proliferative response and cytotoxic T lymphocyte response of mice were detected. The results showed that the fusion gene DNA vaccines induced weaker antibody, but stronger and longer cellular immune responses than single gene DNA vaccines did, which indicated that the envelope and core fusion gene DNA vaccine may be more useful than single gene DNA vaccine for therapy of hepatitis B.

关 键 词：HBV 包膜-核心蛋白融合基因 DNA疫苗 诱导 小鼠 细胞免疫应答 

分 类 号：R512.620.5[医药卫生—内科学] R373.21[医药卫生—临床医学]