猪胴体脂肪沉积性状的QTL定位  

Mapping Quantitative Trait Loci for Fat Deposition in Carcass in Pigs

作　　者：苏玉虹[1] 熊远著[1] 张勤[2] 蒋思文[1] 雷明刚[1] 余雳[1] 郑嵘[1] 邓昌彦[1] 

机构地区：[1]华中农业大学农业部猪遗传育种重点实验室 [2]中国农业大学动物科技学院,北京100094

出　　处：《Acta Genetica Sinica》2002年第8期681-684,共4页

基　　金：973国家重大基础研究项目 (G2 0 0 0 0 1610 5 );湖北省自然科学基金 (2 0 0 0J10 6)资助~~

摘　　要：市场生猪价格的决定因素之一是背膘厚度。因此 ,对养猪行业来说 ,确认影响这一性状的基因组区域就显得非常必要。 1998年 ,由大白和梅山猪杂交繁殖的资源家系 ,随机选留 81头F2 ,全部屠宰。用 4 8个微卫星标记进行1～ 4 ,6～ 7号染色体 (SSC)的扫描。用线性模型最小二乘法对胴体脂肪性状进行区间作图。除SSC2外 ,其余 5条染色体共有 14个QTL影响脂肪沉积 ,其中SSC7上 4个QTL有印记效应存在。平均背膘厚QTL定位于SSC1、4和 7,SSC4平均背膘厚QTL达染色体极显著水平 (P <0 .0 1) ,SSC1和 7上平均背膘厚QTL与多个文献报道结果一致 ,可能是家猪中普遍存在的QTL。花油率QTL定位于SSC6 ,内脂率QTL定位于SSC7。肥肉率等其他 4项脂肪性状定位在SSC3、4和 7。One of the major determining factors in the price of market hogs today is backfat depth. Therefore, identification of regions of the genome affecting this trait is necessary. Gene mapping technologies have provided scientists the necessary reagents to conduct genomewide searches for genes affecting any phenotype determined in part by the genetic makeup of the animal. Over the past few years, several experimental crosses have been used to detect quantitative triat loci (QTL) for fatness and meat quality traits in pigs. In an intercross between Large White and Meishan pigs, 81 F 2 progenies in 1998 were slaughtered and phenotyped for fat deposite traits. F 2 animals, their parents, and their grandparents were typed for molecular markers covering chromosomes (SSC) 1,2,3,4,6 and 7, since previous studies had revealed QTL affecting fatness traits on these chromosomes. Linear model and least square analyses were used for mapping these traits. Furthermore, a QTL accounting for imprinting effects was used. A total of 14 QTL at chromosome wide level were detected on SSC1, 3～4, 6 and 7 for 12 fatness traits. There were evidences for imprinting effects on SSC7 for four traits at 60 cM and nearby. There were QTL for average backfat thickness (BFT) on SSC1,4 and 7. Among them, BFT QTL on SSC1 and 7 may be the common locus for Sus scrofa . QTL for internal fat percentage was on SSC7 and QTL for average sidefat on SSC6.

关 键 词：猪 胴体脂肪沉积性状 QTL定位 

分 类 号：Q953[生物学—动物学]