禾谷缢管蚜体内的病毒结合蛋白基因的克隆与原核表达  

Cloning and Prokaryotic Expression of Viral Binding Protein(VBP) Gene From Endosybiotic Bacterium of Rhopalosiphum padi

作　　者：吴云锋[1] 崔晓锋 林林[1] 周广和[2] 

机构地区：[1]西北农林科技大学,杨凌712100 [2]中国农业科学院植物保护研究所,北京100094

出　　处：《微生物学报》2002年第4期448-452,共5页Acta Microbiologica Sinica

基　　金：国家自然科学基金 ( 39970 4 83) ;国家八六三青年基金 ( 97 1 37)~~

摘　　要：利用一对特异性引物 ,用PCR的方法从禾谷缢管蚜体内扩增出了病毒结合蛋白基因 ,序列测定结果表明其长度为 1 6 4 7bp ,编码 5 4 8个氨基酸 ,与GenBank中的禾谷缢管蚜美国生物型BuchneragroEL NT核苷酸序列同源性为 97% ,氨基酸同源性为 97 4%。构建了 2个原核表达载体并进行表达得到了 6 9kD融合蛋白和 6 3kD的非融合蛋白。Viral binding protein gene from Rhopalosiphum padi Yangling biotype was amplified by PCR method and then cloned.The complete nucleotide sequence of the gene was determined,It has 1647 nucleotides encoding 548 amino acids.Comparison showed this gene has 97% identity on nucleotide level with Buchnera groEL AM gene of Rhopalosiphum padi American biotype,while has 97.4% identity on amino acid level was found between this two genes.The VBP gene was ligated into pBV221 and pET30a expression vector and expressed the aim protein 63kD and 69kD.

关 键 词：禾谷缢管蚜 病毒结合蛋白 原核表达 基因克隆 蚜传病毒 内共生细菌 

分 类 号：Q786[生物学—分子生物学]