高压氧对大鼠脑缺血后NOS阳性神经元的影响  

Influence of hyerbaric oxygen on NOS positive neurons after acute focal cerebral ischemia-reperfusion injury in rats

作　　者：曹义战[1] 晋兴 王伯良[1] 仲月霞[1] 李金声 陆将[1] 何保健[1] 

机构地区：[1]第四军医大学唐都医院急诊科,西安710038 [2]西安高新医院检验科 [3]航空航天医学系高压氧治疗中心

出　　处：《中国急救医学》2002年第8期447-449,共3页Chinese Journal of Critical Care Medicine

摘　　要：目的　探讨一氧化氮合酶 (NOS)阳性细胞在大鼠急性局灶性脑缺血 /再灌注损伤和高压氧 (HBO)治疗后表达的变化。方法　应用血管内细丝栓堵大脑中动脉 (MCA)的局灶性脑缺血大鼠模型 ,利用黄递酶 -NADPH组织化学方法 ,观察MCA缺血 1h ,再灌注 4、11、2 3、71hNOS阳性细胞在视交叉平面梗死区皮层、视前区、纹状体外侧区和纹状体内侧区域分布的变化 ,在以上期间应用 0 2 5MPaHBO于开始缺血后 2、9、2 1、4 5和 6 9h分别治疗 1次 (1h)。结果　脑缺血后 ,在上述区域形态改变的NOS阳性细胞数随着再灌注时间的延长逐渐增多。HBO治疗后 ,各时间点在皮层、视前区和纹状体内侧区 ,形态改变的NOS阳性细胞数明显减少 (P <0 0 5 ) ,而纹状体外侧区无明显变化 (P >0 0 5 )。结论　HBO可明显抑制大鼠急性局灶性脑缺血Objective To investigate the effect of hyperbaric oxygen(HBO) on NOS positive neurons after focal cerebral ischemia reperfusion(IR) injury in rats.Methods Rat model of focal cerebral ischemia induced by intraluminal filament occlusion of middle cerebral artery(MCA) was used.The NOS positive neurons in rat brain were examined after 1h of ischemia with 4,11,23 and 71 h of reperfusion(removed of MCA occlusion) respectively using the NADPH-d histochemistry method.During the reperfusion period,0.25 MPa(ATA)HBO treatment for 1 h each time was applied at 2,9,21,45 and 69 h after the beginning of ischemia.Results The numbers of degeneration NOS positive neurons were increased gradually after MCA occlusion in brain area.After HBO therapy,the numbers of degeneration NOS positive neurons were obviously decreased in cortex,preoptic area and medial striatum (P<0 05),but no obviously descrepancy in the lateral striatum(P>0 05).Conclusion HBO could obviously inhibit the degeneration of NOS positive neurons after focal cerebral ischemia reperfusion injury in rats.

关 键 词：脑缺血 一氧化氮合成酶 一氧化氮 神经元 大鼠 

分 类 号：R459.6[医药卫生—治疗学]