HSV体外感染对IgG产生的抑制作用和TNF及r-IFN对它的影响  

Inhibitory Effect of HSV Infection in Vitro on IgG and Effect of TNF and r-IFN on It

作　　者：曹志惠 陈慎宝[1] 丁如宁[1] 周瑶玺[1] 

机构地区：[1]南京医学院微生物教研室,南京210005

出　　处：《中国病毒学》1991年第3期183-186,共4页Virologica Sinica

基　　金：国家自然科学基金

摘　　要：本文观察了体外HSV-I感染对人淋巴细胞增殖、分化,产生免疫球蛋白的影响。HSV-I能感染PWM刺激的人扁桃体淋巴细胞,并抑制其增殖和免疫球蛋白(IgG)的产生,感染实验组的IgG产量较对照组明显降低(P<0.005)。HSV-I感染后,上清中可检出一定量的病毒,其滴度为10^(3·2)—10^(5·7)TCID50/ml;直接免疫荧光法检出病毒抗原阳性细胞为3％—8.5％。在感染实验系统中加入重组的IFN_a(r-IFN_a)、IFN_γ(r-IFN_γ)和纯化的肿瘤坏死因子(TNF),观察到r-IFN。和TNF均有一定程度抗病毒作用,部分解除HSV-I对IgG产生的抑制作用。和对照组相比,差异显著(P<0.01)。r-IFN_γ对HSV-I的感染和IgG产生几乎无作用(P>0.05),但r-IFN_γ对TNF有协同作用:抑制病毒增殖和解除HSV-I对IgG产生的抑制,比单独用TNF组更为显著(P<0.01)。We successfully established a system in vitro that HSV-Ⅰ infected human tonsillar lymphocytes. Tonsillar lymphocytes prestimulated with PWM(10μg/ml) were infected by living HSV-Ⅰ, so as to their proliferation and IgG synthesis were abated significantly(P<0.005).In the supernatants, the infective titers ofHSV-Ⅰ were about 10^(3·2)—10^(5·7) TCID_(50)/ml,number of viral antigen positive cells about 3％—8％.We found that some lymphokine and monokine can dampen the inhibition of infection of infectious HSV-Ⅰ The effect of purified recombinant IFN-α was stronger than that of IFN-γ(p<0.01). The purified tumor necrosis factor (TNF)also abated alone the inhibition of HSV-Ⅰ markedly, so that IgG synthesis enhanced more highly than that without adding TNF (P<0.01). TNF in combination with IFN-γ caused a larger increasing in IgG synthesis than that observed for TNF alone(P<0.01)or IFN-γ alone(P<0.01). This result showed that the activity of TNF had dramatically synergized with IFN-γ.

关 键 词：单纯疱疹病毒 IGG TNF 干扰素 

分 类 号：R511.990.3[医药卫生—内科学]