γ-羧基谷氨酸在草酸钙尿石形成中的意义  

The Significance of γ-Carboxyglutamic Acid in Urinary Calcium Oxalate Stone Formation

作　　者：鲁德曼[1] 刘继红[1] 周四维[1] 王少刚[1] 蔡丹[1] 庄乾元[1] 杨为民[1] 叶章群[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院泌尿外科,武汉430030

出　　处：《华中科技大学学报（医学版）》2002年第4期403-405,共3页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目 (No.39970 74 0 );武汉市晨光计划资助项目 (No.975 0 0 6 0 98)

摘　　要：为探讨 γ-羧基谷氨酸 (Gla)在草酸钙尿石形成中的作用 ,收集 2 5例尿草酸钙结石患者、 15例健康成人的2 4 h尿液及 10例含钙尿结石患者的结石 ,采用反相高效液相色谱 (HPL C)法检测游离型和蛋白结合型 Gla浓度。结果显示 :结石组和健康对照组尿蛋白结合型 Gla含量分别为 (1.4 4± 0 .32 ) μm ol/ L 及 (1.82± 0 .4 2 ) μmol/ L,两组之间差别有极显著性意义 (P<0 .0 1) ;两组尿液游离型 Gla含量分别为 (5 0 .76± 14 .4 8) μm ol/ L 及 (40 .87± 8.15 ) μmol/ L,两组比较差别有显著性意义 (P<0 .0 5 ) ;结石基质中蛋白结合型 Gla含量为 (72 .11± 2 9.2 2 ) nmol/ 10 0 m g结石。提示 :草酸钙结石患者尿液游离 Gla增加及 Gla结合蛋白中h urine samples were collected from 25 patients with urinary calcium oxalate lithiasis and 15 controls, and 10 stones from 10 patients. Reversed phase high performance liquid chromatography (HPLC) was used to measure γ carboxyglutamic acid (Gla) contents. The result showed that urinary Gla bound protein content was (1 44±0 32) μmol/L in the stone patients and (1 82±0 42) μmol/L in the healthy subjects respectively ( P <0 01). Urinary free Gla content was (50 76±14 48) μmol/L in the patients and (40 87±8 15) μmol/L in the healthy subjects respectively ( P <0 05). The content of stone matrix Gla bound protein was (72 11±29 42) nmol/100 mg stone. It was suggested that the increase of urinary free Gla and the decrease or deficiency of Gla in Gla bound protein may be one of important causes for calcium oxalate stone formation.

关 键 词：草酸钙结石 γ羧基谷氨酸 高效液相色谱 泌尿系结石 

分 类 号：R691.4[医药卫生—泌尿科学]