刀豆素A对小鼠同种胰岛移植的影响  

Effect of concanavalin A on mouse islet transplantation

作　　者：祝希媛[1,2] 何瑾[1,2] 柏平阿 

机构地区：[1]北京友谊医院生理研究室 [2]北京市临床医学研究所病理

出　　处：《中国病理生理杂志》1991年第1期54-57,共4页Chinese Journal of Pathophysiology

摘　　要：本文对刀豆素A(ConA)在小鼠胰岛移植模型上的抗排异作用进行了研究。用胶原酶消化中手挑法获取C57BL/6小鼠的胰岛,将600—700个胰岛移植于链脲霉素复制成糖尿病的BALB/C小鼠肾包膜下,移植胰岛平均存活时间(MST)为14.1±2.5天。实验组用ConA对胰岛预处理后再移植,MST延长为25±5.4天,与对照比较有显著性(P<0.0005):给受鼠注射ConA也使MST显著延长(P<0.05),用ConA对胰岛及受鼠联合处理,MST延长为25.2±4.4天(P<0.0005);实验证明ConA有显著抑制排异反应的作用。对ConA作用机理的初步研究表明:荧光素标记的ConA能与胰岛良好结合,有可能覆盖组织相容抗原,注射ConA后,小鼠淋巴细胞对同系小鼠淋转反应显示一定的抑制作用。To test whether Con A has antirejection effect on mouse islet allograft, 600-700 handpicked islets of C57BL/6 mice was transplanted beneath the kidney capsule of the streptozotocin induced diabetic BALB/C recipients. In control group the mean survival time (MST) of the grafts was 14.1±2.4 days. Treatment with Con A significantly prolong the MST of the islet allografts. In group with Con A treatment of the donor islets prior to the transplantation the MST was 25±5.4 days. The MST increased sigificantly after ip injection of Con A to recipient mice. The combined treatment of islet and recipient with Con A resulted in a prolongation of MST to 25.2±4.4 days. The experiments concerning the mechanisms of the antirejection effect of Con A showed that Con A was able to combine with the islet cell membrane well masking perhaps the histocmpatibility antigen. Furthermore the lymphocytes of mice after i. p Con A injection suppressed the lymphocyte proliferation reaction of the svnergic mice to mitogen.

关 键 词：刀豆球蛋白A 胰岛 淋巴细胞 

分 类 号：Q512.2[生物学—生物化学]